The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Temperature dependence of RNA synthesis parameters in Escherichia coli
Open accessAbstract
For Escherichia coli B/r growing in glucose minimal medium, the following parameters of RNA synthesis remained invariant between 20 and 40 degrees C: RNA polymerase concentration (RNA polymerase/mass), rRNA and tRNA concentration (RNA/mass), RNA polymerase activity (fraction of total RNA polymerase actively engaged in RNA chain elongation), and stable RNA synthesis relative to total RNA synthesis. The following parameters increased 3.4-fold over the same temperature range: rRNA chain elongation rate, guanosine tetraphosphate (ppGpp) concentration, and culture growth rate. Above 40 degrees C, the changes became more complex, and the growth rate began to decrease. The observation that most RNA synthesis parameters are temperature invariant despite the increase of ppGpp suggests that the mechanism of RNA synthesis control by ppGpp, assumed to involve an interaction of RNA polymerase wtih ppGpp, is itself temperature dependent such that, with increasing temperature, higher concentrations of ppGpp are required to affect the RNA polymerase.
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“Because Syn3A still has genes nusG/0840 and nusB/0107, both of which are factors required for rRNA transcription antitermination (Torres et al., 2004), we use an accelerated rate of 85 nt/s for transcription of the RNAP on the rRNA operons (Ryals et al., 1982).”
From Fundamental behaviors emerge from simulations of a living minimal cell (2022) · cites this paper as Ryals et al., 1982
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Cites
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Self-citation
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Cited by
Real-Time Kinetics of Gene Activity in Individual Bacteria
Golding, I., Paulsson, J., Zawilski, S. M., & Cox, E. C. (2005) · Cell · doi:10.1016/j.cell.2005.09.031 · Cited by 1,468
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Self-citationEnergy metabolism controls phenotypes by protein efficiency and allocation
Chen, Y., & Nielsen, J. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1906569116 · Cited by 156
Fundamental behaviors emerge from simulations of a living minimal cell
Thornburg, Z. R., Bianchi, D. M., Brier, T. A., Gilbert, B. R., Earnest, E. E., Melo, M. C. R., Safronova, N., Sáenz, J. P., Cook, A. T., Wise, K. S., et al. (2022) · Cell · doi:10.1016/j.cell.2021.12.025 · Cited by 165
“, 2004), we use an accelerated rate of 85 nt/s for transcription of the RNAP on the rRNA operons (Ryals et al., 1982).”
Kinetic modeling of the genetic information processes in a minimal cell
Thornburg, Z. R., Melo, M. C. R., Bianchi, D., Brier, T. A., Crotty, C., Breuer, M., Smith, H. O., Hutchison, C. A., Glass, J. I., & Luthey-Schulten, Z. (2019) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2019.00130 · Cited by 22
Metadata
- Authors
- Ryals, J., Little, R., & Bremer, H.
- ORCID
- Bremer, H
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1982-08
- Volume
- 151
- Issue
- 2
- Pages
- 879-887
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC220338
- OpenAlex
- W2138963475
- Cited by
- 109
- References
- 28
- Citations by year
- 2026: 2 · 2024: 3 · 2023: 6 · 2022: 4 · 2021: 5 · 2020: 7 · 2019: 5 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 5
- FWCI
- 3.23
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Keywords
- RNA synthesis, temperature dependence, Escherichia coli, RNA polymerase, ribosomal RNA synthesis, tRNA synthesis, ppGpp, RNA polymerase activity, growth rate, glucose minimal medium, RNA synthesis regulation
- MeSH terms
- Bacterial Proteins, Escherichia coli, Guanosine Tetraphosphate, Kinetics, DNA-Directed RNA Polymerases, RNA, Bacterial, RNA, Ribosomal, RNA, Transfer, Temperature
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