The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation
Open accessAbstract
The neutral protease gene of Bacillus subtilis has been cloned, and its nucleotide sequence has been determined. The cloned gene was used to create an in vitro-derived deletion mutation, which was used to replace the wild-type copy of the gene. This deletion, in combination with a deletion of the alkaline protease gene, completely abolished protease production. The loss of the proteases had no detectable effect on growth, morphology, or sporulation.
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Citations
Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
Structural basis of the action of thermolysin and related zinc peptidases
Matthews, B. W. (1988) · Accounts of Chemical Research · doi:10.1021/ar00153a003 · Cited by 687
Westers, L., Westers, H., & Quax, W. J. (2004) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2004.02.011 · Cited by 490
A unique signature identifies a family of zinc‐dependent metallopeptidases
Jongeneel, C. V., Bouvier, J., & Bairoch, A. (1989) · FEBS Letters · doi:10.1016/0014-5793(89)80471-5 · Cited by 461
Protein secretion in Bacillus species
Simonen, M., & Palva, I. (1993) · Microbiological Reviews · doi:10.1128/mmbr.57.1.109-137.1993 · Cited by 390
Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli.
Ikemura, H., Takagi, H., & Inouye, M. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47646-6 · Cited by 335
Metadata
- Authors
- Yang, M. Y., Ferrari, E., & Henner, D. J.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1984-10
- Volume
- 160
- Issue
- 1
- Pages
- 15-21
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1911747505
- Cited by
- 252
- References
- 1
- FWCI
- 21.15
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation. https://app.cochat.ai/papers/10.1128/jb.160.1.15-21.1984
- The paper
- Yang, M. Y., Ferrari, E., & Henner, D. J. (1984). Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation. Journal of Bacteriology. https://doi.org/10.1128/jb.160.1.15-21.1984
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
