The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor
Open accessAbstract
The promoter region of the pColV-K30-encoded operon specifying biosynthesis and transport of the siderophore aerobactin was subjected to deletion analysis to determine the smallest DNA sequence affording iron regulation of a iucA'-'lacZ gene fusion. A 78-base-pair (bp) region containing the main (P1) promoter retained the character of inducibility under iron starvation. A 250-bp fragment carrying this sequence was examined for protection against DNase I by the Fur protein, the product of a gene (fur) required for negative control of several iron-regulated functions. The DNase I footprints, in the presence of various divalent heavy-metal ions added as corepressors, revealed two contiguous binding sites with different lengths and affinities for Fur. Increased concentrations of the protein appeared to elicit formation of repressor oligomers which bind to the upstream and downstream regions of the P1 promoter in a metal-dependent fashion, but with a presently undefined stoichiometry. The primary site for Fur binding spans 31 bp and contains two overlapping symmetry dyads which share the sequence 5'-TCATT-3'. It also contains extensive homology with a 19-bp consensus sequence for iron-regulated genes as deduced from comparison with the fhuA and fepA putative promoter sequences.
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“Consensus sequences bound by Fur in E. coli , P. aeruginosa , V. cholerae and C. jejuni are also AT rich 10 , 17 , 30 , 32 .”
From Mechanistic insights into metal ion activation and operator recognition by the ferric uptake regulator (2015) · cites this paper as [32]
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Self-citationInteractions between DNA-bound repressors govern regulation by the λ phage repressor
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Cited by
Sorted by citations
Herrero, M., de Lorenzo, V., & Timmis, K. N. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.11.6557-6567.1990 · Cited by 1,590
“To achieve expression of the bar gene in a variety of gram-negative bacteria, we placed its transcription under the iron-regulated aerobactin promoter of pColV-K30 (10).”
BackgroundSelf-citationIron and Oxidative Stress in Bacteria
Touati, D. (2000) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1999.1518 · Cited by 915
Opening the Iron Box: Transcriptional Metalloregulation by the Fur Protein
Escolar, L., Pérez-Martín, J., & de Lorenzo, V. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.20.6223-6229.1999 · Cited by 815
Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens
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Role of iron in regulation of virulence genes
Litwin, C. M., & Calderwood, S. B. (1993) · Clinical Microbiology Reviews · doi:10.1128/cmr.6.2.137 · Cited by 651
“coli fur gene itself, raising the possibility that there may be autoregulation of Jifr by iron (44).”
BackgroundMethodInfluential
Metadata
- Authors
- de Lorenzo, V, Wee, S., Herrero, M., & Neilands, J. B.
- Affiliations
- University of California, Berkeley (US)
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1987-06
- Volume
- 169
- Issue
- 6
- Pages
- 2624-2630
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC212138
- OpenAlex
- W2131282911
- Cited by
- 536
- References
- 22
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 7 · 2023: 5 · 2022: 6 · 2021: 8 · 2020: 9 · 2019: 4 · 2018: 8 · 2017: 6 · 2016: 13 · 2015: 12 · 2014: 11 · 2013: 9 · 2012: 21
- FWCI
- 14.06
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministerio de Economía y Competitividad
- National Institutes of Health (1f05tw003577-01)
- U.S. Public Health Service (A104156)
- Fogarty International Center (1F05TWO3577-01-BI-5)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Keywords
- Fur repressor, iron regulation, promoter, DNase I footprinting, repressor binding sites, consensus sequence, siderophore biosynthesis, Escherichia coli
- MeSH terms
- Bacterial Proteins, Chromosome Deletion, Cloning, Molecular, Bacteriocin Plasmids, Escherichia coli, Gene Expression Regulation, Hydroxamic Acids, Iron, Operator Regions, Genetic, Operon, Promoter Regions, Genetic, Recombinant Fusion Proteins, Repressor Proteins, Transcription Factors
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