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Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus
Open accessAbstract
We have previously identified a gene in Staphylococcus aureus, agr, whose activity is required for high-level post-exponential-phase expression of a series of secreted proteins. In this paper, we describe the cloning of this gene in Escherichia coli by using an inserted transposon (Tn551) as a cloning probe. The cloned gene, consisting of a 241-codon open reading frame containing the site of the transposon insertion, was recloned to an S. aureus vector, pSK265, and shown to be functional in S. aureus. Activity was evaluated by determinations of alpha-hemolysin, beta-hemolysin, and toxic shock syndrome toxin-1 production in early-stationary-phase cultures. The cloned gene showed considerable variation with respect to different exoproteins and different host strains compared with the chromosomal agr determinant; this variation could not be attributed to the higher copy number of the cloned gene and probably reflects inapparent subtleties of the regulatory system.
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Citations
Cited by
Slager, J., Kjos, M., Attaiech, L., & Veening, J.-W. (2014) · Cell · doi:10.1016/j.cell.2014.01.068 · Cited by 237
Dale, S. E., Doherty-Kirby, A., Lajoie, G., & Heinrichs, D. E. (2004) · Infection and Immunity · doi:10.1128/iai.72.1.29-37.2004 · Cited by 232
Koch, G., Yepes, A., Förstner, K. U., Wermser, C., Stengel, S. T., Modamio, J., Ohlsen, K., Foster, K. R., & Lopez, D. (2014) · Cell · doi:10.1016/j.cell.2014.06.046 · Cited by 211
“…(Hla, Hlb, and Hlg), proteases, lipases, enterotoxins, superantigens, and urease while at the same time indirectly downregulating the extracellular matrix and adhesin proteins responsible for cell aggregation and biofilm formation (Boles and Horswill, 2008; Peng et al., 1988; Recsei et al., 1986).”
Clements, M. O., Watson, S. P., & Foster, S. J. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.13.3898-3903.1999 · Cited by 185
Lyristis, M., Bryant, A. E., Sloan, J., Awad, M. M., Nisbet, I. T., Stevens, D. L., & Rood, J. I. (1994) · Molecular Microbiology · doi:10.1111/j.1365-2958.1994.tb01063.x · Cited by 165
Metadata
- Authors
- Peng, H. L., Novick, R. P., Kreiswirth, B., Kornblum, J., & Schlievert, P.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1988-09
- Volume
- 170
- Issue
- 9
- Pages
- 4365-4372
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1790927204
- Cited by
- 566
- References
- 0
- FWCI
- 11.14
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus. https://app.cochat.ai/papers/10.1128/jb.170.9.4365-4372.1988
- The paper
- Peng, H. L., Novick, R. P., Kreiswirth, B., Kornblum, J., & Schlievert, P. (1988). Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus. Journal of Bacteriology. https://doi.org/10.1128/jb.170.9.4365-4372.1988
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