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New thermosensitive plasmid for gram-positive bacteria
Open accessAbstract
We isolated a replication-thermosensitive mutant of the broad-host-range replicon pWV01. The mutant pVE6002 is fully thermosensitive above 35 degrees C in both gram-negative and gram-positive bacteria. Four clustered mutations were identified in the gene encoding the replication protein of pVE6002. The thermosensitive derivative of the related plasmid pE194 carries a mutation in the analogous region but not in the same position. Derivatives of the thermosensitive plasmid convenient for cloning purposes have been constructed. The low shut-off temperature of pVE6002 makes it a useful suicide vector for bacteria which are limited in their own temperature growth range. Using pVE6002 as the delivery vector for a transposon Tn10 derivative in Bacillus subtilis, we observed transposition frequencies of about 1%.
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Citations
Cited by
Efficient insertional mutagenesis in lactococci and other gram-positive bacteria
Maguin, E., Prévost, H., Ehrlich, S. D., & Gruss, A. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.3.931-935.1996 · Cited by 486
Self-citationThomas, V. C., Thurlow, L. R., Boyle, D., & Hancock, L. E. (2008) · Journal of Bacteriology · doi:10.1128/jb.00314-08 · Cited by 302
Doran, K. S., Engelson, E. J., Khosravi, A., Maisey, H. C., Fedtke, I., Equils, O., Michelsen, K. S., Arditi, M., Peschel, A., & Nizet, V. (2005) · Journal of Clinical Investigation · doi:10.1172/jci23829 · Cited by 231
Wall, T., Båth, K., Britton, R. A., Jonsson, H., Versalovic, J., & Roos, S. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.01502-06 · Cited by 131
Gene transfer to Clostridium cellulolyticum ATCC 35319
Jennert, K. C. B., Tardif, C., Young, D. I., & Young, M. (2000) · Microbiology · doi:10.1099/00221287-146-12-3071 · Cited by 112
Metadata
- Authors
- Maguin, E., Duwat, P., Hege, T., Ehrlich, D., & Gruss, A.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1992-09
- Volume
- 174
- Issue
- 17
- Pages
- 5633-5638
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1548162677
- Cited by
- 443
- References
- 0
- FWCI
- 5.92
- Type
- journal article
- Language
- en
- Open access
- green
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- Maguin, E., Duwat, P., Hege, T., Ehrlich, D., & Gruss, A. (1992). New thermosensitive plasmid for gram-positive bacteria. Journal of Bacteriology. https://doi.org/10.1128/jb.174.17.5633-5638.1992
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