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Cloning and sequencing of sarA of Staphylococcus aureus, a gene required for the expression of agr
Open accessAbstract
To evaluate the effect of a sar mutation on the agr locus, Northern (RNA) blotting was performed to determine the levels of RNAIII, the agr regulatory molecule, in two isogenic pairs of Staphylococcus aureus strains. Our results demonstrated that RNAIII was either significantly diminished or absent in both sar mutants compared with the parents. The RNAIII level was partially restored in sar mutants complemented with an intact sar gene (designated sarA). Additionally, we were able to complement selected sar phenotypes with a plasmid carrying RNAIII (pRN6735). These studies suggest that the sarA gene is necessary for the optimal expression of agr. The sarA gene of strain RN450 was subsequently cloned and sequenced. Sequence analysis revealed an open reading frame of 372 bp with a predicted molecular size of 14,718 Da and a deduced pI of 8.52. The deduced protein sequence has a predominance of charged residues (33%) and shares sequence similarity with the virF gene of Shigella flexneri.
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Cited by
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Quorum Sensing in Staphylococci
Novick, R. P., & Geisinger, E. (2008) · Annual Review of Genetics · doi:10.1146/annurev.genet.42.110807.091640 · Cited by 958
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Vuong, C., & Otto, M. (2002) · Microbes and Infection · doi:10.1016/s1286-4579(02)01563-0 · Cited by 708
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Jenul, C., & Horswill, A. R. (2019) · Microbiology Spectrum · doi:10.1128/microbiolspec.gpp3-0031-2018 · Cited by 549
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Metadata
- Authors
- Cheung, A. L., & Projan, S. J.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1994-07
- Volume
- 176
- Issue
- 13
- Pages
- 4168-4172
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1562798956
- Cited by
- 200
- References
- 0
- FWCI
- 7.19
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Cloning and sequencing of sarA of Staphylococcus aureus, a gene required for the expression of agr. https://app.cochat.ai/papers/10.1128/jb.176.13.4168-4172.1994
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- Cheung, A. L., & Projan, S. J. (1994). Cloning and sequencing of sarA of Staphylococcus aureus, a gene required for the expression of agr. Journal of Bacteriology. https://doi.org/10.1128/jb.176.13.4168-4172.1994
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