Two divergent MET10 genes, one from Saccharomyces cerevisiae and one from Saccharomyces carlsbergensis, encode the alpha subunit of sulfite reductase and specify potential binding sites for FAD and NADPH
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The yeast assimilatory sulfate reductase is a complex enzyme that is responsible for conversion of sulfite into sulfide. To obtain information on the nature of this enzyme, we isolated and sequenced the MET10 gene of Saccharomyces cerevisiae and a divergent MET10 allele from Saccharomyces carlsbergensis. The polypeptides deduced from the identically sized open reading frames (1,035 amino acids) of both MET10 genes have molecular masses of around 115 kDa and are 88% identical to each other. The transcript of S. cerevisiae MET10 has a size comparable to that of the open reading frame and is transcriptionally repressed by methionine in a way similar to that seen for other MET genes of S. cerevisiae. Distinct homology was found between the putative MET10-encoded polypeptide and flavin-interacting parts of the sulfite reductase flavoprotein subunit (encoded by cysJ) from Escherichia coli and several other flavoproteins. A significant N-terminal homology to pyruvate flavodoxin oxidoreductase (encoded by nifJ) from Klebsiella pneumoniae, together with a lack of obvious flavin mononucleotide-binding motifs in the MET10 deduced amino acid sequence, suggests that the yeast assimilatory sulfite reductase is a distinct type of sulfite reductase.
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Sulfite reductase is a heterotetramer of two α and two β subunits encoded by MET10 and MET5.
“Sulfite reductase is a heterotetramer, consisting of two αand two β-subunits, which are encoded by MET10 and MET5 genes, respectively (Kobayashi and Yoshimoto 1982, Hansen et al. 1994, Sutherland et al. 2003).”
From Yeast and bacterial modulation of wine aroma and flavour (2005) · cites this paper as Hansen et al. 1994
The introduction reports that earlier results suggested an α2β2 structure, and the paper identifies MET10 as encoding the α subunit. It says the size of MET5 mRNA suggests MET5 might encode the β subunit; it does not establish that assignment or the subunit arrangement as fact. (LLM, conf=0.87)
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Yeast and bacterial modulation of wine aroma and flavour
SWIEGERS, J. H., BARTOWSKY, E. J., HENSCHKE, P. A., & PRETORIUS, I. S. (2005) · Australian Journal of Grape and Wine Research · doi:10.1111/j.1755-0238.2005.tb00285.x · Cited by 1,216 · see citing works
- Authors
- Hansen, J., Cherest, H., & Kielland-Brandt, M. C.
- Affiliations
- Carlsberg Laboratory (DK)
- Department of Yeast Genetics, Carlsberg Laboratory, Copenhagen Valby, Denmark.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1994-10
- Volume
- 176
- Issue
- 19
- Pages
- 6050-6058
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC196824
- OpenAlex
- W1943939928
- Cited by
- 56
- References
- 0
- Citations by year
- 2026: 1 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 4 · 2016: 1 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 1.27
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Bacteriology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nitrogen and Sulfur Effects on Brassica
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Keywords
- Saccharomyces cerevisiae, Saccharomyces carlsbergensis, alpha subunit, FAD binding site, NADPH binding site, methionine repression, flavoprotein, sulfate assimilation
- MeSH terms
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Enzyme Repression, Flavin-Adenine Dinucleotide, Flavoproteins, Genes, Fungal, Methionine, Molecular Sequence Data, NADP, Oxidation-Reduction, Protein Conformation, RNA, Messenger, Saccharomyces, Saccharomyces cerevisiae, Sulfites, Gene Expression Regulation, Fungal, Sequence Homology, Amino Acid, Sequence Analysis, DNA, Oxidoreductases Acting on Sulfur Group Donors, Sulfite Reductase (NADPH)
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