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Role of the rfe gene in the biosynthesis of the Escherichia coli O7-specific lipopolysaccharide and other O-specific polysaccharides containing N-acetylglucosamine
Open accessAbstract
We report that rfe mutants of wild-type strains of Escherichia coli O7, O18, O75, and O111 did not express O-specific polysaccharide unless the rfe mutation was complemented by a cloned rfe gene supplied in a plasmid. The O polysaccharides in these strains are known to have N-acetylglucosamine (GlcNAc) in their O repeats. In addition, in vitro transferase assays with bacterial membranes from either the O7 wild-type strain or its isogenic rfe mutant showed that GlcNAc is the first carbohydrate added onto the lipid acceptor in the assembly of the O7 repeat and that this function is inhibited by tunicamycin. Our results indicate that the rfe gene product is a general requirement for the synthesis of O polysaccharides containing GlcNAc.
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Citations
Cited by
Raetz, C. R. H., & Whitfield, C. (2002) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.71.110601.135414 · Cited by 4,404
Samuel, G., & Reeves, P. (2003) · Carbohydrate Research · doi:10.1016/j.carres.2003.07.009 · Cited by 517
The structures of Escherichia coli O-polysaccharide antigens
Stenutz, R., Weintraub, A., & Widmalm, G. (2006) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2006.00016.x · Cited by 406
Biosynthesis of lipopolysaccharide O antigens
Whitfield, C. (1995) · Trends in Microbiology · doi:10.1016/s0966-842x(00)88917-9 · Cited by 300
Metadata
- Authors
- Alexander, D. C., & Valvano, M. A.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1994-11
- Volume
- 176
- Issue
- 22
- Pages
- 7079-7084
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1584292656
- Cited by
- 170
- References
- 0
- FWCI
- 1.88
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Role of the rfe gene in the biosynthesis of the Escherichia coli O7-specific lipopolysaccharide and other O-specific polysaccharides containing N-acetylglucosamine. https://app.cochat.ai/papers/10.1128/jb.176.22.7079-7084.1994
- The paper
- Alexander, D. C., & Valvano, M. A. (1994). Role of the rfe gene in the biosynthesis of the Escherichia coli O7-specific lipopolysaccharide and other O-specific polysaccharides containing N-acetylglucosamine. Journal of Bacteriology. https://doi.org/10.1128/jb.176.22.7079-7084.1994
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
