The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
A Mutation in a Purported Regulatory Gene Affects Control of Sterol Uptake in Saccharomyces cerevisiae
Open accessAbstract
Aerobically growing wild-type strains of Saccharomyces cerevisiae are unable to take exogenously supplied sterols from media. This aerobic sterol exclusion is vitiated under anaerobic conditions, in heme-deficient strains, and under some conditions of impaired sterol synthesis. Mutants which can take up sterols aerobically in heme-competent cells have been selected. One of these mutations, designated upc2-1, gives a pleiotropic phenotype in characteristics as diverse as aerobic accumulation of sterols, total lipid storage, sensitivity to metabolic inhibitors, response to altered sterol structures, and cation requirements. During experiments designed to ascertain the effects of various cations on yeast with sterol alterations, it was observed that upc2-1 was hypersensitive to Ca2+. Using resistance to Ca2+ as a screening vehicle, we cloned UPC2 and showed that it is YDR213W, an open reading frame on chromosome IV. This belongs to a fungal regulatory family containing the Zn(II)2Cys6 binuclear cluster DNA binding domain. The single guanine-to-adenine transition in upc2-1 gives a predicted amino acid change from glycine to aspartic acid. The regulatory defect explains the semidominance and pleiotropic effects of upc2-1.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“ERG11 is transcriptionally regulated by the zinc cluster transcription factor Upc2.”
From Gain-of-Function Mutations in UPC2 Are a Frequent Cause of ERG11 Upregulation in Azole-Resistant Clinical Isolates of Candida albicans (2012) · cites this paper as [4]
Experimental — accuracy not yet measuredIn the citing paper
“The relevant mutation (upc2-1) [19] is a change of a glycine to an aspartate residue located at position (G888D) in the carboxy-terminus of ScUpc2.”
From The Candida glabrata Upc2A transcription factor is a global regulator of antifungal drug resistance pathways (2021) · cites this paper as [19]
In the citing paper
“The S. cerevisiae UPC2-1 allele, from which we derived the G898D UPC2A, was originally isolated on the basis of permitting aerobic sterol uptake [19].”
From The Candida glabrata Upc2A transcription factor is a global regulator of antifungal drug resistance pathways (2021) · cites this paper as [19]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Sikorski, R. S., & Hieter, P. (1989) · Genetics · doi:10.1093/genetics/122.1.19 · Cited by 8,590
Cold Spring Harbor Laboratory; Cold Spring Harbor, NY
Cavalier-Smith, T. (1980) · FEBS Letters · doi:10.1016/0014-5793(80)80323-1 · Cited by 6,317
Improved method for high efficiency transformation of intact yeast cells.
Gietz, D., Jean, A. S., Woods, R. A., & Schiestl, R. (1992) · Nucleic Acids Research · doi:10.1093/nar/20.6.1425 · Cited by 3,379
Methods in Yeast Genetics: A Laboratory Course Manual
Rose, M. D., Winston, F., & Hieter, P. (1990) · Medical Entomology and Zoology · Cited by 1,894
Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.
Rothstein, R. (1991) · Methods in Enzymology · doi:10.1016/0076-6879(91)94022-5 · Cited by 1,283
Berben, G., Dumont, J. E., Gilliquet, V., Bolle, P. A., & Hilger, F. (1991) · Yeast · doi:10.1002/yea.320070506 · Cited by 339
Phagocytic killing ofCandida albicansby different murine effector cells
Baccarini, M., Blasi, E., Puccetti, P., & Bistoni, F. (1983) · Medical Mycology · doi:10.1080/00362178385380431 · Cited by 339
PHYSIOLOGICAL IMPLICATIONS OF STEROL BIOSYNTHESIS IN YEAST
Parks, L. W., & Casey, W. M. (1995) · Annual Review of Microbiology · doi:10.1146/annurev.mi.49.100195.000523 · Cited by 336
Self-citationEvolution of a Fungal Regulatory Gene Family: The Zn(II)2Cys6 Binuclear Cluster DNA Binding Motif
Todd, R. B., & Andrianopoulos, A. (1997) · Fungal Genetics and Biology · doi:10.1006/fgbi.1997.0993 · Cited by 295
Cited by
Sorted by citations
Items 101–123 of 123
Ecm22 and Upc2 regulate yeast mating through control of expression of the mating genes PRM1 and PRM4
Höfken, T. (2017) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2017.10.005 · Cited by 6
The Ins and Outs of Azole Antifungal Drug Resistance: Molecular Mechanisms of Transport
Zavřel, M., Esquivel, B. D., & White, T. C. (2014) · Springer eBooks · doi:10.1007/978-1-4939-0667-3_29-1 · Cited by 6
Snoek, I. S. I., Tai, S. L., Pronk, J. T., Steensma, H. Y., & Daran, J.-M. G. (2010) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2010.00622.x · Cited by 6
The anti‐fungal β‐sitosterol targets the yeast oxysterol‐binding protein Osh4
Moosavi, B., Liu, S., Wang, N., Zhu, X., & Yang, G. (2019) · Pest Management Science · doi:10.1002/ps.5568 · Cited by 5
Cell integrity limits ploidy in budding yeast
Barker, J., Murray, A. W., & Bell, S. P. (2025) · G3 Genes Genomes Genetics · doi:10.1093/g3journal/jkae286 · Cited by 5
Niimi, M. (2010) · Nippon Ishinkin Gakkai Zasshi · doi:10.3314/jjmm.51.79 · Cited by 4
Vu, B. G., Stamnes, M. A., Li, Y., Rogers, P. D., & Moye-Rowley, S. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.05.07.443069 · Cited by 4
Resistance to Antifungal Drugs
Sanglard, D. (2010) · Springer eBooks · doi:10.1007/978-1-4419-6640-7_9 · Cited by 3
Hervay, N. T., Bencova, A., Valachovič, M., Morvová, M., & Gbelská, Y. (2020) · Yeast · doi:10.1002/yea.3527 · Cited by 3
Candida glabrata YPK2 is a multidrug susceptibility locus
Šimoničová, L., Conway, T. P., Krüger, T., Brakhage, A. A., & Moye-Rowley, S. (2026) · Genetics · doi:10.1093/genetics/iyag176 · Cited by 1
Identification and regulation of genes involved in anaerobic growth of Saccharomyces cerevisiae
Snoek, I. S. I. (2007) · Leiden Repository (Leiden University) · Cited by 1
Blosser, S. J. (2013) · Montana State University ScholarWorks (Montana State University)
Whybrew, J. M. (2010) · Purdue e-Pubs (Purdue University System)
Structural, physical and genetic interactions of the i-AAA protease Yme1 in Saccharomyces cerevisiae
Engmann, T. (2009) · Kölner Universitäts PublikationsServer (Universität zu Köln)
This is to certify that the thesis/dissertation prepared
Hoblitzell, A., Mukhopadhyay, S., & Xia, Y. (2010)
ERG11-Mediated Azole Resistance in Candida albicans
Flowers, S. A. (2013) · doi:10.21007/etd.cghs.2013.0096
Zhang, W., Guo, J., Wang, Z., Li, Y., Meng, X., Shen, Y., & Liu, W. (2021) · Figshare · doi:10.6084/m9.figshare.13544627
Song, H. Y., Lee, G. W., Jung, Y. H., & Lee, J. W. (2026) · Frontiers in Chemical Engineering · doi:10.3389/fceng.2026.1833599
Meng, F.-Z., Wei, W.-K., Wu, M., Cai, M.-Z., Wang, Y., Zhao, L. D., Meng, S., Yin, L., Schnabel, G., Yin, W., et al. (2026) · Advanced Science · doi:10.1002/advs.77796 · Cited by 1
Metadata
- Authors
- Crowley, J. H., Leak, F. W., Shianna, K. V., Tove, S., & Parks, L. W.
- Affiliations
- North Carolina State University (US)
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1998-08-15
- Volume
- 180
- Issue
- 16
- Pages
- 4177-4183
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC107415
- OpenAlex
- W2159654630
- Cited by
- 123
- References
- 26
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 5 · 2020: 7 · 2019: 3 · 2018: 4 · 2017: 5 · 2015: 3 · 2014: 4 · 2013: 10 · 2012: 2
- FWCI
- 1.17
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed Central (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Veterans Affairs (TPP 97-003)
- Agricultural Research Service
- Army Research Office
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Keywords
- sterol uptake, UPC2, Saccharomyces cerevisiae, heme deficiency, sterol biosynthesis, pleiotropic mutations, calcium sensitivity, fat deposition, semi-dominant mutation
- MeSH terms
- Amino Acid Sequence, Base Sequence, Calcium, Fungal Proteins, Genes, Fungal, Genes, Regulator, Genetic Complementation Test, Molecular Sequence Data, Mutation, Saccharomyces cerevisiae, Sterols, Trans-Activators, Open Reading Frames, Saccharomyces cerevisiae Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
