The DOI matches its Crossref record.
Visualization of Phospholipid Domains in Escherichia coli by Using the Cardiolipin-Specific Fluorescent Dye 10- N -Nonyl Acridine Orange
Open accessAbstract
Cardiolipin (CL)-specific fluorescent dye 10- N -nonyl-acridine orange (NAO) was used to visualize CL distribution in Escherichia coli cells of different phospholipid compositions. In a filamentous mutant containing only anionic phospholipids, green fluorescent spots were observed along the filaments at approximately regular intervals. Three-dimensional image reconstruction obtained by optical sectioning and a deconvolution algorithm revealed NAO-binding domains in the plane of the cell membrane. Substantial red fluorescence emission of bound NAO supported labeling of CL-containing domains. These structures were not found in mutants deficient in CL biosynthesis. The domains were also observed mostly in the septal region and on the poles in cells of normal size with wild-type phospholipid composition.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
FtsZ and the division of prokaryotic cells and organelles
Margolin, W. (2005) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1745 · Cited by 631
Hartmann, M., Berditsch, M., Hawecker, J., Ardakani, M. F., Gerthsen, D., & Ulrich, A. S. (2010) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00124-10 · Cited by 543
Antimicrobial Peptides Targeting Gram-Positive Bacteria
Malanovic, N., & Lohner, K. (2016) · Pharmaceuticals · doi:10.3390/ph9030059 · Cited by 420
Schlame, M. (2008) · Journal of Lipid Research · doi:10.1194/jlr.r700018-jlr200 · Cited by 388
Bacterial osmoregulation: a paradigm for the study of cellular homeostasis.
Wood, J. M. (2011) · Annual Review of Microbiology · doi:10.1146/annurev-micro-090110-102815 · Cited by 308
Metadata
- Authors
- Mileykovskaya, E., & Dowhan, W.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2000-02-15
- Volume
- 182
- Issue
- 4
- Pages
- 1172-1175
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2101040320
- Cited by
- 432
- References
- 0
- FWCI
- 3.72
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Visualization of Phospholipid Domains in Escherichia coli by Using the Cardiolipin-Specific Fluorescent Dye 10- N -Nonyl Acridine Orange. https://app.cochat.ai/papers/10.1128/jb.182.4.1172-1175.2000
- The paper
- Mileykovskaya, E., & Dowhan, W. (2000). Visualization of Phospholipid Domains in Escherichia coli by Using the Cardiolipin-Specific Fluorescent Dye 10- N -Nonyl Acridine Orange. Journal of Bacteriology. https://doi.org/10.1128/jb.182.4.1172-1175.2000
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
