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In Vivo Effect of NusB and NusG on rRNA Transcription Antitermination
Open accessAbstract
Similarities between lambda and rRNA transcription antitermination have led to suggestions that they involve the same Nus factors. However, direct in vivo confirmation that rRNA antitermination requires all of the lambda Nus factors is lacking. We have therefore analyzed the in vivo role of NusB and NusG in rRNA transcription antitermination and have established that both are essential for it. We used a plasmid test system in which reporter gene mRNA was measured to monitor rRNA antiterminator-dependent bypass of a Rho-dependent terminator. A comparison of terminator read-through in a wild-type Escherichia coli strain and that in a nusB::IS10 mutant strain determined the requirement for NusB. In the absence of NusB, antiterminator-dependent terminator read-through was not detected, showing that NusB is necessary for rRNA transcription antitermination. The requirement for NusG was determined by comparing rRNA antiterminator-dependent terminator read-through in a strain overexpressing NusG with that in a strain depleted of NusG. In NusG-depleted cells, termination levels were unchanged in the presence or absence of the antiterminator, demonstrating that NusG, like NusB, is necessary for rRNA transcription antitermination. These results imply that NusB and NusG are likely to be part of an RNA-protein complex formed with RNA polymerase during transcription of the rRNA antiterminator sequences that is required for rRNA antiterminator-dependent terminator read-through.
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“Because Syn3A still has genes nusG/0840 and nusB/0107, both of which are factors required for rRNA transcription antitermination (Torres et al., 2004), we use an accelerated rate of 85 nt/s for transcription of the RNAP on the rRNA operons (Ryals et al., 1982).”
From Fundamental behaviors emerge from simulations of a living minimal cell (2022) · cites this paper as Torres et al., 2004
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rRNA Transcription in Escherichia coli
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Kang, J. Y., Mooney, R. A., Nedialkov, Y. A., Saba, J., Mishanina, T. V., Artsimovitch, I., Landick, R. C., & Darst, S. A. (2018) · Cell · doi:10.1016/j.cell.2018.05.017 · Cited by 171
Fundamental behaviors emerge from simulations of a living minimal cell
Thornburg, Z. R., Bianchi, D. M., Brier, T. A., Gilbert, B. R., Earnest, E. E., Melo, M. C. R., Safronova, N., Sáenz, J. P., Cook, A. T., Wise, K. S., et al. (2022) · Cell · doi:10.1016/j.cell.2021.12.025 · Cited by 165
“Because Syn3A still has genes nusG/0840 and nusB/0107, both of which are factors required for rRNA transcription antitermination (Torres et al., 2004), we use an accelerated rate of 85 nt/s for transcription of the RNAP on the rRNA operons (Ryals et al.”
Metadata
- Authors
- Torres, M., Balada, J.-M., Zellars, M., Squires, C., & Squires, C. L.
- Affiliations
- King Faisal Specialist Hospital & Research Centre (SA)
- Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111
- Tufts University (US)
- Corresponding author
- Squires, Craig
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2004-03
- Volume
- 186
- Issue
- 5
- Pages
- 1304-1310
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC344418
- OpenAlex
- W2164650717
- Cited by
- 68
- References
- 40
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 4 · 2018: 5 · 2017: 1 · 2016: 4 · 2015: 4 · 2014: 2 · 2013: 2 · 2012: 3
- FWCI
- 2.32
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM024751, GM24751)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- NusB, NusG, rRNA transcription, antitermination, Rho-dependent transcription termination, RNA polymerase, protein-RNA complex, Escherichia coli
- MeSH terms
- Escherichia coli, Mutation, Peptide Elongation Factors, Rho Factor, RNA, Messenger, RNA, Ribosomal, Terminator Regions, Genetic, Transcription Factors, Transcription, Genetic, Blotting, Western, Gene Expression Regulation, Bacterial, Escherichia coli Proteins
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