Genetic Diversity and Virulence Profiles of Escherichia coli Isolates Causing Spontaneous Bacterial Peritonitis and Bacteremia in Patients with Cirrhosis
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Among patients with cirrhosis, infections caused by Escherichia coli organisms that translocate from the gut are a frequent and severe complication. One hundred ten E. coli isolates from 110 cirrhotic patients with spontaneous bacterial peritonitis and/or spontaneous bacteremia were characterized for their phylogenetic group and virulence genotype (34 extraintestinal virulence factor genes). Genetic relatedness was investigated by enterobacterial repetitive intergenic consensus sequence type 2 (ERIC-2) PCR typing and multilocus sequence typing. Phylogenetic groups A, B1, B2, and D accounted for 24%, 4%, 48%, and 24% of the population, respectively. Overall, 68 distinct ERIC-2 profiles were encountered. Eleven clonal groups, represented by multiple isolates (2 to 11) from the same sequence type (ST) or sequence type complex, were identified. These clonal groups accounted for 54 (49%) isolates overall. Membership in one of these clonal groups was more frequent among B2 isolates than non-B2 isolates (67% versus 32%, P < 0.001). The most frequent sequence types were ST95 ( n = 13) and ST73 ( n = 8), followed by the ST14 and ST10 complexes ( n = 7). ST131 and ST69 were represented by three isolates each. Clonal group-associated isolates exhibited a greater prevalence of 11 virulence genes, including pap elements, than the other isolates. However, no association between clonal groups and host factors, type of infection, or mortality was observed. In conclusion, E. coli isolates causing spontaneous bacterial peritonitis and bacteremia in cirrhotic patients are genetically diverse. However, approximately half of the isolates belong to familiar clonal groups and exhibit extensive virulence profiles that may be associated with greater invasive potential.
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Gram-negative bacteria are the most adept at translocating to mesenteric lymph nodes and are the most frequent cause of spontaneous bacterial infections in cirrhosis patients.
“Gram-negative bacteria have been found to be the most adept at translocating to the mesenteric lymph nodes and are the most frequent cause of spontaneous bacterial infections in patients with cirrhosis105–107.”
From The gut microbiome and liver cancer: mechanisms and clinical translation (2017) · cites this paper as [107]
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References 40 · Cited by 71
- Authors
- Bert, F., Johnson, J. R., Ouattara, B., Leflon-Guibout, V., Johnston, B., Marcon, E., Valla, D., Moreau, R., & Nicolas-Chanoine, M.-H.
- Affiliations
- Service de Microbiologie, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris (AP-HP), Clichy, France
- University of Minnesota (US)
- Inserm (FR)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2010-08
- Volume
- 48
- Issue
- 8
- Pages
- 2709-2714
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC2916625
- OpenAlex
- W2157403593
- Cited by
- 71
- References
- 40
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 8 · 2020: 2 · 2019: 8 · 2018: 2 · 2017: 5 · 2016: 5 · 2015: 5 · 2014: 10 · 2013: 3 · 2012: 11
- FWCI
- 4.06
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Enterobacteriaceae and Cronobacter Research
- Keywords
- Escherichia coli, spontaneous bacterial peritonitis, liver cirrhosis, genetic diversity, virulence genes, phylogenetic groups, multilocus sequence typing, clonal groups, pap gene, ST95, ST73
- MeSH terms
- Adult, Aged, DNA, Bacterial, Escherichia coli, Escherichia coli Infections, Female, Genotype, Humans, Liver Cirrhosis, Male, Middle Aged, Peritonitis, Phylogeny, Genetic Variation, Virulence, Bacterial Typing Techniques, Prevalence, Cluster Analysis, Polymerase Chain Reaction, DNA Fingerprinting, Bacteremia, Sequence Analysis, DNA, Molecular Epidemiology, Escherichia coli Proteins, Virulence Factors
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