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Cited by 47Cervical Cancer and HPV ResearchReproductive tract infections researchGenital Health and Disease

Confirmatory Real-Time PCR Assay for Human Papillomavirus (HPV) Type 52 Infection in Anogenital Specimens Screened for HPV Infection with the Linear Array HPV Genotyping Test

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Coutlée, François, Rouleau, Danielle, Ghattas, Georges, Hankins, Catherine, Vézina, Sylvie, Coté, Pierre, Macleod, John, de Pokomandy, Alexandra +5 more·2007·Journal of Clinical Microbiology
DOI 10.1128/jcm.01145-07PMC PMC2168527
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Abstract

A novel real-time PCR assay for detection of human papillomavirus type 52 (HPV-52) DNA (RT-52) was evaluated on 265 anogenital samples. RT-52 had a sensitivity of 98.4% and a specificity of 100% compared to conventional HPV-52 typing assays, including hybridization of PGMY products with an HPV-52-specific probe and PCR sequencing of HPV-52 E6.

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Authors
Coutlée, F., Rouleau, D., Ghattas, G., Hankins, C., Vézina, S., Coté, P., Macleod, J., de Pokomandy, A., Money, D., Walmsley, S., Voyer, H., Brassard, P., & Franco, E.
Affiliations
  • Laboratoire de Virologie Moléculaire, Centre de Recherche, et Départements de Microbiologie et Infectiologie, de Médecine Familiale et Gastro-entérologie, Hôpital Notre-Dame du Centre Hospitalier de l'Université de Montréal, Montréal, Québec, Canada
  • Hôpital Notre-Dame (CA)
  • Institut National de Santé Publique du Québec (CA)
  • Clinique Médicale l'Actuel, Montréal, Québec, Canada
  • Clinique Médicale du Quartier-Latin, Montréal, Québec, Canada
  • McGill University (CA)
  • University of British Columbia (CA)
  • University of Toronto (CA)
ORCID
Venue
Journal of Clinical Microbiology
Publisher
American Society for Microbiology
Published
2007-11
Volume
45
Issue
11
Pages
3821-3823
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
DOI
10.1128/jcm.01145-07
PMC
PMC2168527
OpenAlex
W2156424776
Cited by
47
References
0
Citations by year
2026: 1 · 2025: 2 · 2024: 1 · 2022: 4 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 3 · 2012: 4
FWCI
3.22
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Clinical Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Styrelsen för Internationellt Utvecklingssamarbete
  • Ontario HIV Treatment Network
  • National Cancer Institute
Topics
  • Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Health Sciences › Medicine › Surgery › Genital Health and Disease
Keywords
real-time PCR, HPV genotyping, assay sensitivity and specificity
MeSH terms
Anal Canal, DNA, Viral, Female, Genitalia, Genotype, Humans, Male, Sensitivity and Specificity, Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Papillomaviridae, Papillomavirus Infections

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