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Rapid Detection of Mycobacterium tuberculosis Complex and Rifampin Resistance in Smear-Negative Clinical Samples by Use of an Integrated Real-Time PCR Method
Open accessAbstract
Sixty-four of 85 (75.3%) smear-negative respiratory ( n = 78) and nonrespiratory ( n = 7) samples with positive cultures of Mycobacterium tuberculosis complex (MTC) were detected by the GeneXpert system using the Xpert MTB/RIF assay (GX). In addition, GX found rpoB mutations in all six of the rifampin-resistant strains detected. The test was negative in 20 culture-negative and 20 nontuberculous culture-positive samples (100% specificity). GX offers high potential for the diagnosis of tuberculosis due to its capacity for direct detection of MTC, its rapidity, and its simplicity.
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Rapid Molecular Detection of Tuberculosis and Rifampin Resistance
Boehme, C. C., Nabeta, P., Hillemann, D., Nicol, M. P., Shenai, S., Krapp, F., Allen, J., Tahirli, R., Blakemore, R., Rustomjee, R., et al. (2010) · New England Journal of Medicine · doi:10.1056/nejmoa0907847 · Cited by 2,309
Evaluation of the analytical performance of the Xpert MTB/RIF assay
Blakemore, R., Story, E., Helb, D., Kop, J., Banada, P., Owens, M. R., Chakravorty, S., Jones, M., & Alland, D. (2010) · Journal of Clinical Microbiology · doi:10.1128/jcm.00128-10 · Cited by 485
Cited by
Boehme, C. C., Nicol, M. P., Nabeta, P., Michael, J. S., Gotuzzo, E., Tahirli, R., Gler, M. T., Blakemore, R., Worodria, W., Gray, C., et al. (2011) · The Lancet · doi:10.1016/s0140-6736(11)60438-8 · Cited by 1,013
Zeka, A. N., Tasbakan, S., & Cavusoglu, C. (2011) · Journal of Clinical Microbiology · doi:10.1128/jcm.05434-11 · Cited by 362
Clinical validation of Xpert MTB/RIF for the diagnosis of extrapulmonary tuberculosis
Tortoli, E., Russo, C., Piersimoni, C., Mazzola, E., Dal Monte, P., Pascarella, M., Borroni, E., Mondo, A., Piana, F., Scarparo, C., et al. (2012) · European Respiratory Journal · doi:10.1183/09031936.00176311 · Cited by 322
Xpert MTB/RIF and Xpert MTB/RIF Ultra for pulmonary tuberculosis and rifampicin resistance in adults
Horne, D. J., Kohli, M., Zifodya, J. S., Schiller, I., Dendukuri, N., Tollefson, D., Schumacher, S. G., Ochodo, E. A., Pai, M., & Steingart, K. R. (2019) · Cochrane Database of Systematic Reviews · doi:10.1002/14651858.cd009593.pub4 · Cited by 267
Rapid molecular TB diagnosis: evidence, policy making and global implementation of Xpert MTB/RIF
Weyer, K., Mirzayev, F., Migliori, G. B., Van Gemert, W., D'Ambrosio, L., Zignol, M., Floyd, K., Centis, R., Cirillo, D. M., Tortoli, E., et al. (2013) · European Respiratory Journal · doi:10.1183/09031936.00157212 · Cited by 256
Metadata
- Authors
- Moure, R., Muñoz, L., Torres, M., Santin, M., Martín, R., & Alcaide, F.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2011-03
- Volume
- 49
- Issue
- 3
- Pages
- 1137-1139
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2155790306
- Cited by
- 165
- References
- 2
- FWCI
- 11.14
- Type
- journal article
- Language
- en
- Open access
- green
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- Moure, R., Muñoz, L., Torres, M., Santin, M., Martín, R., & Alcaide, F. (2011). Rapid Detection of Mycobacterium tuberculosis Complex and Rifampin Resistance in Smear-Negative Clinical Samples by Use of an Integrated Real-Time PCR Method. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.01831-10
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.