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Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography
Open accessAbstract
A standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an Aminex HPX-87 column. The acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 N NaOH. The presence of fumaric acid in culture extracts of Peptostreptococcus anaerobius was confirmed by gas chromatography-mass spectrometry analysis of the trapped eluent fractions from the high-performance liquid chromatography column.
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Citations
Cited by
Marilley, L. (2004) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(03)00304-0 · Cited by 420
Short chain fatty acids and their receptors: new metabolic targets
Layden, B. T., Angueira, A. R., Brodsky, M., Durai, V., & Lowe, W. L. (2013) · Translational Research · doi:10.1016/j.trsl.2012.10.007 · Cited by 357
Interaction between colonic acetate and propionate in humans
Wolever, T. M. S., Spadafora, P., & Eshuis, H. (1991) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/53.3.681 · Cited by 332
Park, D. H., Laivenieks, M., Guettler, M. V., Jain, M. K., & Zeikus, J. G. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.7.2912-2917.1999 · Cited by 281
Acosta, A., Camilleri, M., Shin, A., Nord, S. L., O'Neill, J., Gray, A. V., Lueke, A. J., Donato, L. J., Burton, D. D., Szarka, L. A., et al. (2016) · Clinical and Translational Gastroenterology · doi:10.1038/ctg.2016.32 · Cited by 88
Metadata
- Authors
- Guerrant, G. O., Lambert, M. A., & Moss, C. W.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1982-08
- Volume
- 16
- Issue
- 2
- Pages
- 355-360
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W1655139143
- Cited by
- 163
- References
- 0
- FWCI
- 4.07
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Guerrant, G. O., Lambert, M. A., & Moss, C. W. (1982). Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.16.2.355-360.1982
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.