Detection and identification of Mycobacterium tuberculosis by DNA amplification
Open accessAbstract
The polymerase chain reaction (PCR) was used to identify mycobacterial DNA sequences in uncultured clinical specimens. Two oligonucleotide primers derived from the sequence of a gene that codes for the 65-kilodalton antigen of Mycobacterium tuberculosis amplified DNA from all 11 species of mycobacteria tested. Amplified DNAs of nontuberculosis mycobacteria were found to be approximately 20 to 40 bases shorter than those from M. tuberculosis and Mycobacterium bovis BCG. DNA equivalent to that present in as few as 40 M. tuberculosis cells either alone or in the presence of DNA equivalent to that in 10(6) human cells could be detected. Results from analysis of cultured bacteria and clinical specimens showed PCR was sensitive and specific both in detecting mycobacteria and in differentiating M. tuberculosis and BCG from other species of mycobacteria. The PCR method with the primers reported here may become a useful tool in the early and rapid detection of mycobacterial infections in uncultured clinical specimens.
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PCR amplification of specific DNA sequences is a novel tool for detection of infectious organisms and has been applied to detect mycobacterial DNA sequences in several materials.
“Recently, the amplification of specific DNA sequences by polymerase chain reaction (PCR) is a novel tool for the detection of different infectious organisms and has already been applied to detect mycobacterial DNA sequences in several materials (Pao et al., 1990; Plikaytis et al., 1991; Sritharan et al., 1991; Ghossein et al., 1992 ; Saboor et al., 1992; Popper et al., 1994).”
From Application of PCR from the fine needle aspirates for the diagnosis of cervical tuberculous lymphadenitis. (1996) · cites this paper as Pao et al., 1990
Theoretically only two organisms of AFB are enough to detect successfully with PCR amplification.
“Theoretically only two organisms of AFB are enough to detect successfully with PCR amplification (Pao et al., 1990; Plikaytis et al., 1991).”
From Application of PCR from the fine needle aspirates for the diagnosis of cervical tuberculous lymphadenitis. (1996) · cites this paper as Pao et al., 1990
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References 13 · Cited by 208
- Authors
- Pao, C. C., Yen, T. S., You, J. B., Maa, J. S., Fiss, E. H., & Chang, C. H.
- Affiliations
- Chang Gung Memorial Hospital (TW)
- Department of Biochemistry, Chang Gung Medical College and Memorial Hospital, Taipei, Taiwan.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1990-09
- Volume
- 28
- Issue
- 9
- Pages
- 1877-1880
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC268070
- OpenAlex
- W1932920735
- Cited by
- 208
- References
- 13
- Citations by year
- 2026: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 5 · 2013: 6 · 2012: 3
- FWCI
- 19.71
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Keywords
- Mycobacterium tuberculosis, nontuberculous mycobacteria, Mycobacterium bovis BCG, primer sequences
- MeSH terms
- Base Sequence, DNA, Bacterial, Gene Amplification, Humans, Molecular Sequence Data, Mycobacterium tuberculosis, DNA Probes, Polymerase Chain Reaction
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