The DOI matches its Crossref record.
Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory
Open accessAbstract
Clinical isolates of Mycobacterium spp. were identified by direct sequence determination of 16S rRNA gene fragments amplified by polymerase chain reaction. Identification was based on a hypervariable region within the 16S rRNA gene in which mycobacterial species are characterized by species-specific nucleotide sequences. A manually aligned data base including the signature sequences of 52 species of mycobacteria easily allowed rapid and correct identification. The results of this study demonstrate that polymerase chain reaction-mediated direct sequence determination can be used as a rapid and reliable method for the identification of mycobacteria in the clinical laboratory. In addition, the prompt recognition of previously undescribed species is now feasible.
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Cited by
Griffith, D. E., Aksamit, T., Brown-Elliott, B. A., Catanzaro, A., Daley, C., Gordin, F., Holland, S. M., Horsburgh, R., Huitt, G., Iademarco, M. F., et al. (2007) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200604-571st · Cited by 6,110
Springer, B., Stockman, L., Teschner, K., Roberts, G. D., & Böttger, E. C. (1996) · Journal of Clinical Microbiology · doi:10.1128/jcm.34.2.296-303.1996 · Cited by 371
Self-citationChakravorty, S., Sen, M. K., & Tyagi, J. S. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.9.4357-4362.2005 · Cited by 282
Troesch, A., Nguyen, H., Miyada, C. G., Desvarenne, S., Gingeras, T. R., Kaplan, P. M., Cros, P., & Mabilat, C. (1999) · Journal of Clinical Microbiology · doi:10.1128/jcm.37.1.49-55.1999 · Cited by 272
Honoré-Bouakline, S., Vincensini, J. P., Giacuzzo, V., Lagrange, P. H., & Herrmann, J. L. (2003) · Journal of Clinical Microbiology · doi:10.1128/jcm.41.6.2323-2329.2003 · Cited by 168
Metadata
- Authors
- Kirschner, P., Springer, B., Vogel, U., Meier, A., Wrede, A., Kiekenbeck, M., Bange, F. C., & Böttger, E. C.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1993-11
- Volume
- 31
- Issue
- 11
- Pages
- 2882-2889
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W1955005444
- Cited by
- 479
- References
- 0
- FWCI
- 17.97
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. https://app.cochat.ai/papers/10.1128/jcm.31.11.2882-2889.1993
- The paper
- Kirschner, P., Springer, B., Vogel, U., Meier, A., Wrede, A., Kiekenbeck, M., Bange, F. C., & Böttger, E. C. (1993). Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.31.11.2882-2889.1993
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
