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Cited by 513

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Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day

Open access
Gautom, R K·1997·Journal of Clinical Microbiology

Abstract

Genomic DNA patterns generated by pulsed-field gel electrophoresis are highly specific for different strains of an organism and have significant value in epidemiologic investigations of infectious-disease outbreaks. Unfortunately, time-consuming and tedious specimen processing is an inherent problem which limits the use of this powerful technology as a real-time epidemic investigational tool. Here, I describe a rapid method to improve the response time and provide specific bacterial strain identification for the typing of Escherichia coli O157:H7 and other gram-negative organisms in a single day.

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Crossref · 10.1128/jcm.35.11.2977-2980.1997

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Metadata

Authors
Gautom, R. K.
Venue
Journal of Clinical Microbiology
Published
1997-11
Volume
35
Issue
11
Pages
2977-2980
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
OpenAlex
W2104549414
Cited by
513
References
0
FWCI
6.53
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day. https://app.cochat.ai/papers/10.1128/jcm.35.11.2977-2980.1997
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Gautom, R. K. (1997). Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.35.11.2977-2980.1997

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.