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Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model
Open accessAbstract
A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet.
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Citations
Cited by
PCR inhibitors - occurrence, properties and removal
Schrader, C., Schielke, A., Ellerbroek, L., & Johne, R. (2012) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2012.05384.x · Cited by 2,118
Ongeng, D., Geeraerd, A. H., Springael, D., Ryckeboer, J., Muyanja, C., & Mauriello, G. (2015) · Critical Reviews in Microbiology · doi:10.3109/1040841x.2013.829415 · Cited by 73
Metadata
- Authors
- Monteiro, L., Bonnemaison, D., Vekris, A., Petry, K. G., Bonnet, J., Vidal, R., Cabrita, J., & Mégraud, F.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1997-04
- Volume
- 35
- Issue
- 4
- Pages
- 995-998
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2101960580
- Cited by
- 551
- References
- 0
- FWCI
- 15.63
- Type
- journal article
- Language
- en
- Open access
- bronze
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How to cite
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- CoChat. (2026). Citation check: Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. https://app.cochat.ai/papers/10.1128/jcm.35.4.995-998.1997
- The paper
- Monteiro, L., Bonnemaison, D., Vekris, A., Petry, K. G., Bonnet, J., Vidal, R., Cabrita, J., & Mégraud, F. (1997). Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.35.4.995-998.1997
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
