The DOI matches its Crossref record.
Development of PCR Assays for Species- and Type-Specific Identification of Pasteurella multocida Isolates
Open accessAbstract
Genomic subtractive hybridization of closely related Pasteurella multocida isolates has generated clones useful in distinguishing hemorrhagic septicemia-causing type B strains from other P. multocida serotypes. Oligonucleotide primers designed during the sequencing of these clones have proved valuable in the development of PCR assays for rapid species- and type-specific detection of P. multocida and of type B:2 in particular. This study demonstrated that the primer pair designed from the sequence of the clone 6b (KTT72 and KTSP61) specifically amplified a DNA fragment from types B:2, B:5, and B:2,5 P. multocida and that the primers KMT1T7 and KMT1SP6 produced an amplification product unique to all P. multocida isolates analyzed. It was also shown that PCR amplification performed directly on bacterial colonies or cultures represents an extremely rapid, sensitive method of P. multocida identification.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Townsend, K. M., Boyce, J. D., Chung, J. Y., Frost, A. J., & Adler, B. (2001) · Journal of Clinical Microbiology · doi:10.1128/jcm.39.3.924-929.2001 · Cited by 494
Self-citationMyers, J. M., & Myers, C. R. (2000) · Journal of Bacteriology · doi:10.1128/jb.182.1.67-75.2000 · Cited by 203
Metadata
- Authors
- Townsend, K. M., Frost, A. J., Lee, C. W., Papadimitriou, J. M., & Dawkins, H. J. S.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1998-04
- Volume
- 36
- Issue
- 4
- Pages
- 1096-1100
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2106000944
- Cited by
- 423
- References
- 0
- FWCI
- 3.52
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Development of PCR Assays for Species- and Type-Specific Identification of Pasteurella multocida Isolates. https://app.cochat.ai/papers/10.1128/jcm.36.4.1096-1100.1998
- The paper
- Townsend, K. M., Frost, A. J., Lee, C. W., Papadimitriou, J. M., & Dawkins, H. J. S. (1998). Development of PCR Assays for Species- and Type-Specific Identification of Pasteurella multocida Isolates. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.36.4.1096-1100.1998
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
