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hsp65 Sequencing for Identification of Rapidly Growing Mycobacteria
Open accessAbstract
Partial sequencing of the hsp65 gene was used for the identification of rapidly growing mycobacteria (RGM). A 441-bp fragment (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. Böttger, and T. Bodmer, J. Clin. Microbiol. 31:175–178, 1993) was amplified and sequenced by an automated fluorescence-based method involving capillary electrophoresis. Type strains of 10 RGM species were first studied. Each species had a unique nucleotide sequence, distinguishing it clearly from the other species. A panel of strains from the four main RGM species responsible for human infections, Mycobacterium abscessus , Mycobacterium chelonae , Mycobacterium fortuitum , and Mycobacterium peregrinum , was also studied. There were few sequence differences within each of these species (<2% of bases were different from the type strain sequence), and they had no effect on species assignment. hsp65 sequencing unambiguously differentiated M. chelonae and M. abscessus , two species difficult to identify by classical methods and 16S rRNA gene sequencing. The devised procedure is a rapid and reliable tool for the identification of RGM species.
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Cited by
Genetic Dissection of Immunity to Mycobacteria: The Human Model
Casanova, J.-L., & Abel, L. (2002) · Annual Review of Immunology · doi:10.1146/annurev.immunol.20.081501.125851 · Cited by 991
Non-tuberculous mycobacteria and the rise of Mycobacterium abscessus
Johansen, M. D., Herrmann, J.-L., & Kremer, L. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-020-0331-1 · Cited by 822
A Global Survey of Mycobacterial Diversity in Soil
Walsh, C. M., Gebert, M. J., Delgado-Baquerizo, M., Maestre, F. T., & Fierer, N. (2019) · Applied and Environmental Microbiology · doi:10.1128/aem.01180-19 · Cited by 111
Metadata
- Authors
- Ringuet, H., Akoua-Koffi, C., Honore, S., Varnerot, A., Vincent, V., Berche, P., Gaillard, J. L., & Pierre-Audigier, C.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1999-03
- Volume
- 37
- Issue
- 3
- Pages
- 852-857
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W1547900160
- Cited by
- 304
- References
- 0
- FWCI
- 7.14
- Type
- journal article
- Language
- en
- Open access
- green
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- Ringuet, H., Akoua-Koffi, C., Honore, S., Varnerot, A., Vincent, V., Berche, P., Gaillard, J. L., & Pierre-Audigier, C. (1999). hsp65 Sequencing for Identification of Rapidly Growing Mycobacteria. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.37.3.852-857.1999
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