Rapid Detection of West Nile Virus from Human Clinical Specimens, Field-Collected Mosquitoes, and Avian Samples by a TaqMan Reverse Transcriptase-PCR Assay

Lanciotti, Robert S.; Kerst, Amy J.; Nasci, Roger S.; Godsey, Marvin S.; Mitchell, Carl J.; Savage, Harry M.; Komar, Nicholas; Panella, Nicholas A.; Allen, Becky C.; Volpe, Kate E.; Davis, Brent S.; Roehrig, John T.

doi:10.1128/jcm.38.11.4066-4071.2000

Rapid Detection of West Nile Virus from Human Clinical Specimens, Field-Collected Mosquitoes, and Avian Samples by a TaqMan Reverse Transcriptase-PCR Assay

The authors report on the development and application of a rapid TaqMan assay for the detection of West Nile (WN) virus in a variety of human clinical specimens and field-collected specimens. Oligonucleotide primers and FAM- and TAMRA-labeled WN virus-specific probes were designed by using the nu…