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Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay
Open accessAbstract
A multiplex PCR assay was developed by using primers to the fiber gene that could differentiate human adenovirus (Ad) species A through F in a single amplification reaction. The assay correctly identified the species of all 49 recognized Ad prototype strains as well as 180 geographically and temporally diverse Ad field isolates. Ad serotype 6 (Ad6) (species C), Ad16 (species B), Ad31 (species A), and Ad40 and Ad41 (species F) could also be distinguished by amplicon size within each respective species. In comparison, a previously described Ad species-specific multiplex PCR assay that used primers to the Ad hexon gene gave equivocal results with several serotypes of species B, whereas our multiplex assay amplified all species B serotypes equally well. Our multiplex PCR assay will permit rapid, accurate, and cost-effective classification of Ad isolates.
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Cited by
Rapid and quantitative detection of human adenovirus DNA by real‐time PCR
Heim, A., Ebnet, C., Harste, G., & Pring‐Åkerblom, P. (2003) · Journal of Medical Virology · doi:10.1002/jmv.10382 · Cited by 820
Performance of forty-one microbial source tracking methods: A twenty-seven lab evaluation study
Boehm, A. B., Van De Werfhorst, L. C., Griffith, J. F., Holden, P. A., Jay, J. A., Shanks, O. C., Wang, D., & Weisberg, S. B. (2013) · Water Research · doi:10.1016/j.watres.2012.12.046 · Cited by 313
Wang, Y., Guo, Y., Kuang, Q., Pu, X., Ji, Y., Zhang, Z., & Li, M. (2015) · Journal of Computer-Aided Molecular Design · doi:10.1007/s10822-014-9827-y · Cited by 69
Metadata
- Authors
- Xu, W., McDonough, M. C., & Erdman, D. D.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2000-11
- Volume
- 38
- Issue
- 11
- Pages
- 4114-4120
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2170115663
- Cited by
- 281
- References
- 0
- FWCI
- 5.15
- Type
- journal article
- Language
- en
- Open access
- green
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- Xu, W., McDonough, M. C., & Erdman, D. D. (2000). Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.38.11.4114-4120.2000
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