Quality Control Limits for Broth Microdilution Susceptibility Tests of Ten Antifungal Agents
Open accessAbstract
Broth microdilution susceptibility tests of Candida species have now been standardized by the National Committee for Clinical Laboratory Standards (NCCLS). An eight-laboratory collaborative study was carried out in order to document reproducibility of tests of Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258 by the NCCLS method. Replicate broth microdilution tests were used to define control limits for 24- and 48-h MICs of amphotericin B, flucytosine, fluconazole, voriconazole, ketoconazole, itraconazole, caspofungin (MK 0991), ravuconazole (BMS 207147), posaconazole (SCH 56592), and LY 303366.
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For the five molds and the yeast isolate, >99% of the inocula were within the target ranges (0.4 × 10^6 to 5 × 10^6 CFU/ml for molds and 1 × 10^6 to 5 × 10^6 CFU/ml for C. krusei).
“for the five molds and the yeast isolate, >99% of the inocula were within the target ranges (0.4 × 106 to 5 × 106 CFU/ml [molds] and 1 × 106 to 5 × 106 CFU/ml[C. krusei]) or similar reproducibility as in previous collaborative studies (92 to 95%) (1, 2, 7, 8).”
From Quality Control Guidelines for Amphotericin B, Itraconazole, Posaconazole, and Voriconazole Disk Diffusion Susceptibility Tests with Nonsupplemented Mueller-Hinton Agar (CLSI M51-A Document) for Nondermatophyte Filamentous Fungi (2011) · cites this paper as [1]
The paper’s inoculum-density passage (page not specified in the indexed copy) reports testing two yeast control strains, not five molds and one yeast. For C. krusei, it reports counts of 0.2 × 10³ to 2.5 × 10³ CFU/ml after inoculation, contrary to the claimed range of 1 × 10⁶ to 5 × 10⁶ CFU/ml; it does not report the claimed >99% figure. (LLM, conf=0.84)
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References 10 · Cited by 211
- Authors
- Barry, A. L., Pfaller, M. A., Brown, S. D., Espinel-Ingroff, A., Ghannoum, M. A., Knapp, C., Rennie, R. P., Rex, J. H., & Rinaldi, M. G.
- Affiliations
- The Clinical Microbiology Institute, Wilsonville, Oregon 1 ;
- University of Iowa (US)
- Virginia Commonwealth University (US)
- University Center Reference Laboratory for Medical Mycology, Cleveland, Ohio 4 ;
- Trek Diagnostic Systems, Inc., Westlake, Ohio 5 ;
- National Center for Mycology, Edmonton, Alberta, Canada 6 ; and
- The University of Texas Health Science Center at Houston (US)
- The University of Texas at San Antonio Health Science Center (US)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2000
- Volume
- 38
- Issue
- 9
- Pages
- 3457-3459
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC87406
- OpenAlex
- W1774456129
- Cited by
- 211
- References
- 10
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 5 · 2019: 1 · 2017: 2 · 2016: 5 · 2015: 6 · 2014: 4 · 2013: 2 · 2012: 5
- FWCI
- 15.70
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- quality control limits, broth microdilution, antifungal susceptibility testing, Candida parapsilosis, Candida krusei, reproducibility, minimum inhibitory concentration, amphotericin B, 5-fluorocytosine, fluconazole, voriconazole, ketoconazole, itraconazole, caspofungin, ravuconazole, posaconazole, antifungal agents
- MeSH terms
- Antifungal Agents, Candida, Laboratories, Microbial Sensitivity Tests, Quality Control, Reproducibility of Results
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