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Single-Tube Single-Enzyme Reverse Transcriptase PCR Assay for Detection of Bovine Viral Diarrhea Virus in Pooled Bovine Serum
Open accessAbstract
A reverse transcriptase PCR (RT-PCR) was developed for use as a diagnostic screening test for the detection of bovine viral diarrhea virus (BVDV) in pooled bovine serum samples. Individual serum samples from 60 dairy cattle herds located in Pennsylvania were evaluated by the microplate virus isolation method, and pooled sera were analyzed by RT-PCR. RT-PCR was sensitive and specific and detected a single viremic serum sample in up to 100 pooled serum samples. RT-PCR analysis of pooled sera provides a rapid and cost-effective method for the screening of cattle herds for the presence of animals persistently infected with BVDV.
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“BVDV genomes were also detected in the transtracheal washes of both slaughtered and necropsied animals, some of which showed no clinical signs [132].”
From Strategies for Bovine Respiratory Disease (BRD) Diagnosis and Prognosis: A Comprehensive Overview (2024) · cites this paper as [132]
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Research works printed but not linked: 21
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Strategies for Bovine Respiratory Disease (BRD) Diagnosis and Prognosis: A Comprehensive Overview
Kamel, M. S., Davidson, J. L., & Verma, M. S. (2024) · Animals · doi:10.3390/ani14040627 · Cited by 76
“BVDV genomes were also detected in the transtracheal washes of both slaughtered and necropsied animals, some of which showed no clinical signs [132].”
Background
Metadata
- Authors
- Weinstock, D., Bhudevi, B., & Castro, A. E.
- Affiliations
- Pennsylvania State University (US)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2001-01
- Volume
- 39
- Issue
- 1
- Pages
- 343-346
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC87727
- OpenAlex
- W2146932940
- Cited by
- 100
- References
- 0
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 1 · 2022: 4 · 2021: 2 · 2020: 6 · 2019: 5 · 2018: 6 · 2017: 1 · 2016: 6 · 2014: 3 · 2013: 8 · 2012: 5
- FWCI
- 4.21
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Disease Management and Epidemiology
- Keywords
- bovine viral diarrhea virus, reverse transcription PCR, pooled serum, herd screening, persistent infection, dairy cattle, sensitivity and specificity
- MeSH terms
- Animals, Diarrhea Viruses, Bovine Viral, Bovine Virus Diarrhea-Mucosal Disease, Cattle, RNA, Viral, Sensitivity and Specificity, Viremia, Reverse Transcriptase Polymerase Chain Reaction
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