Rapid Detection of Enterovirus RNA in Cerebrospinal Fluid Specimens with a Novel Single-Tube Real-Time Reverse Transcription-PCR Assay

Verstrepen, Walter A.; Kuhn, Sofie; Kockx, Mark M.; Van De Vyvere, Martine E.; Mertens, An H.

doi:10.1128/jcm.39.11.4093-4096.2001

Rapid Detection of Enterovirus RNA in Cerebrospinal Fluid Specimens with a Novel Single-Tube Real-Time Reverse Transcription-PCR Assay

A single-tube real-time reverse transcription-PCR (RT-PCR) assay for enterovirus detection in cerebrospinal fluid (CSF) was developed based on a fluorogenic probe and primers directed to highly conserved sequences in the 5′ untranslated region of the enterovirus genome. Quantitative detection of …