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Nucleic Acid Sequence-Based Amplification Assays for Rapid Detection of West Nile and St. Louis Encephalitis Viruses
Open accessAbstract
The development and application of nucleic acid sequence-based amplification (NASBA) assays for the detection of West Nile (WN) and St. Louis encephalitis (SLE) viruses are reported. Two unique detection formats were developed for the NASBA assays: a postamplification detection step with a virus-specific internal capture probe and electrochemiluminescence (NASBA-ECL assay) and a real-time assay with 6-carboxyfluorescein-labeled virus-specific molecular beacon probes (NASBA-beacon assay). The sensitivities and specificities of these NASBA assays were compared to those of a newly described standard reverse transcription (RT)-PCR and TaqMan assays for SLE virus and to a previously published TaqMan assay for WN virus. The NASBA assays demonstrated exceptional sensitivities and specificities compared to those of virus isolation, the TaqMan assays, and standard RT-PCR, with the NASBA-beacon assay yielding results in less than 1 h. These assays should be of utility in the diagnostic laboratory to complement existing diagnostic testing methodologies and as a tool in conducting flavivirus surveillance in the United States.
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LAMP and NASBA have been studied in various CNS infections and might especially be useful in laboratories with limited resources.
“LAMP and NASBA have been studied in various CNS infections and might especially be useful in laboratories with limited resources (26–31).”
From Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections (2024) · cites this paper as [30]
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References 25 · Cited by 267
Research works printed but not linked: 2
- Chan A. B. Fox J. D. NASBA and other transcription-based amplification methods for research and diagnostic microbiology.Rev. Med. Microbiol.101999185196
- Monath T. P. Heinz F. X. Flaviviruses Fields virology 3rd ed. Fields B. N. 1 1996 978 984 Lippincott-Raven Publishers Philadelphia, Pa
Metadata
- Authors
- Lanciotti, R. S., & Kerst, A. J.
- Affiliations
- Centers for Disease Control and Prevention (US)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2001-12
- Volume
- 39
- Issue
- 12
- Pages
- 4506-4513
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC88574
- OpenAlex
- W2169133350
- Cited by
- 267
- References
- 25
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 4 · 2023: 6 · 2022: 7 · 2021: 10 · 2020: 4 · 2019: 8 · 2018: 7 · 2017: 4 · 2016: 7 · 2015: 8 · 2014: 8 · 2013: 11 · 2012: 9
- FWCI
- 19.76
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Centers for Disease Control and Prevention
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- NASBA, West Nile virus, St. Louis encephalitis virus, flavivirus detection, electrochemiluminescence assay, molecular beacons, rapid virus detection, flavivirus surveillance, sensitivity and specificity
- MeSH terms
- Animals, Bird Diseases, Birds, Chlorocebus aethiops, Encephalitis Virus, St. Louis, Encephalitis, St. Louis, Culicidae, RNA, Viral, Sensitivity and Specificity, Time Factors, Vero Cells, West Nile Fever, West Nile virus, Molecular Probes, Taq Polymerase, Reverse Transcriptase Polymerase Chain Reaction, Self-Sustained Sequence Replication
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