Inoculum Standardization for Antifungal Susceptibility Testing of Filamentous Fungi Pathogenic for Humans
Open accessAbstract
Two methods of inoculum preparation for filamentous fungi were compared: counting with a hematocytometer and spectrophotometric adjustment. One hundred eighty-two filamentous fungi pathogenic for humans were used. Colony counts were done for all inoculum preparations. The agreement between the hematocytometer counts and the colony counts (CFU per milliliter) was 97.2%. The reproducibility between the hematocytometer counts and the colony counts by means of an intraclass correlation coefficient was 0.70. Pearson's correlation index for hematocytometer counts versus colony counts was 0.56, whereas that for optical density versus colony counts was 0.008. Both methods can be used for inoculum size adjustment. However, the use of the spectrophotometric method requires that each species be standardized separately.
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Numerous studies have shown the importance of inoculum preparation by haemocytometer counting for reproducible and suitable preparation independent of the color and size of conidia.
“Numerous studies showed the importance of inoculum preparation by haemocytometer counting for reproducible and suitable preparation independent of the color and size of conidia [68], [73], [74].”
From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [73]
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Methods for in vitro evaluating antimicrobial activity: A review
Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016) · Journal of Pharmaceutical Analysis · doi:10.1016/j.jpha.2015.11.005 · Cited by 7,264 · see citing works
Mellado, E., Garcia-Effron, G., Alcázar-Fuoli, L., Melchers, W. J. G., Verweij, P. E., Cuenca-Estrella, M., & Rodríguez-Tudela, J. L. (2007) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01092-06 · Cited by 490 · see citing works
Self-citationAntimicrobial effect of resveratrol on dermatophytes and bacterial pathogens of the skin
Chan, M. M.-Y. (2002) · Biochemical Pharmacology · doi:10.1016/s0006-2952(01)00886-3 · Cited by 287 · see citing works
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Self-citationArendrup, M. C., Kahlmeter, G., Guinea, J., & Meletiadis, J. (2021) · Clinical Microbiology and Infection · doi:10.1016/j.cmi.2020.08.042 · Cited by 150 · see citing works
Antifungal susceptibility testing in<i>Aspergillus</i>spp. according to EUCAST methodology
Lass-Flörl, C., Cuenca-Estrella, M., Denning, D. W., & Rodriguez-Tudela, J. L. (2006) · Medical Mycology · doi:10.1080/13693780600779401 · Cited by 69 · see citing works
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- Authors
- Petrikkou, E., Rodrı́guez-Tudela, J. L., Cuenca-Estrella, M., Gómez, A., Molleja, A., & Mellado, E.
- Affiliations
- Instituto de Salud Carlos III (ES)
- <!--label omitted: 1-->Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 Majadahonda, Spain
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2001-04
- Volume
- 39
- Issue
- 4
- Pages
- 1345-1347
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC87937
- OpenAlex
- W2098804733
- Cited by
- 134
- References
- 1
- Citations by year
- 2026: 6 · 2025: 5 · 2024: 16 · 2023: 6 · 2022: 5 · 2021: 10 · 2020: 6 · 2019: 6 · 2018: 3 · 2017: 3 · 2016: 4 · 2015: 8 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Instituto de Salud Carlos III
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Keywords
- inoculum standardization, antifungal susceptibility testing, filamentous fungi, human pathogenic fungi, colony count, reproducibility
- MeSH terms
- Antifungal Agents, Aspergillus, Mitosporic Fungi, Fusarium, Humans, Microbial Sensitivity Tests, Mycoses, Spectrophotometry, Colony Count, Microbial, Scedosporium
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