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Cited by 366

The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.

Rapid Pulsed-Field Gel Electrophoresis Protocol for Subtyping of Campylobacter jejuni

Open access
Ribot, Efrain M., Fitzgerald, Collette, Kubota, Kristy, Swaminathan, Bala, Barrett, Timothy J.·2001·Journal of Clinical Microbiology

Abstract

We developed a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Campylobacter isolates based on the standardized protocols used by PulseNet laboratories for the subtyping of other food-borne bacterial pathogens. Various combinations of buffers, reagents, reaction conditions (e.g., cell suspension concentration, lysis time, lysis temperature, and restriction enzyme concentration), and electrophoretic parameters were evaluated in an effort to devise a protocol that is simple, rapid, and robust. PFGE analysis of Campylobacter isolates can be completed in 24 to 30 h using this protocol, whereas the most widely used current protocols require 3 to 4 days to complete. Comparison of PFGE patterns obtained in six laboratories showed that subtyping results obtained using this protocol are highly reproducible.

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Crossref · 10.1128/jcm.39.5.1889-1894.2001

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Metadata

Authors
Ribot, E. M., Fitzgerald, C., Kubota, K., Swaminathan, B., & Barrett, T. J.
Venue
Journal of Clinical Microbiology
Published
2001-05
Volume
39
Issue
5
Pages
1889-1894
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
OpenAlex
W2117139877
Cited by
366
References
13
FWCI
20.80
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Rapid Pulsed-Field Gel Electrophoresis Protocol for Subtyping of Campylobacter jejuni. https://app.cochat.ai/papers/10.1128/jcm.39.5.1889-1894.2001
The paper
Ribot, E. M., Fitzgerald, C., Kubota, K., Swaminathan, B., & Barrett, T. J. (2001). Rapid Pulsed-Field Gel Electrophoresis Protocol for Subtyping of Campylobacter jejuni. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.39.5.1889-1894.2001

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.