Tetrazolium Microplate Assay as a Rapid and Inexpensive Colorimetric Method for Determination of Antibiotic Susceptibility of Mycobacterium tuberculosis
Open accessAbstract
The emergence of multidrug-resistant tuberculosis underscores the need for low-cost, rapid methods to determine the susceptibility of Mycobacterium tuberculosis to antibiotics. A new, rapid, easily read, and inexpensive colorimetric method with a tetrazolium indicator performs this determination as quickly and accurately as the more expensive Alamar Blue technique.
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A rapid and inexpensive colorimetric method based on Alamar blue and MTT has been successfully used for determining MICs of first-line drugs in DST of M. tuberculosis.
“A rapid and inexpensive colorimetric method based on the oxidation-reduction indicators Alamar blue and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] have been successfully used for determining MICs of first-line drugs in DST of Mycobacterium tuberculosis (1, 9, 20, 31, 38, 49).”
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Martin, A., Camacho, M., Portaels, F., & Palomino, J. C. (2003) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.47.11.3616-3619.2003 · Cited by 306 · see citing works
Torres-Romero, D., Jiménez, I. A., Rojas, R., Gilman, R. H., López, M., & Bazzocchi, I. L. (2011) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2011.02.034 · Cited by 54 · see citing works
- Authors
- Caviedes, L., Delgado, J., & Gilman, R. H.
- Affiliations
- Universidad Peruana Cayetano Heredia (PE)
- Asociación Benéfica PRISMA, Lima, Peru
- Johns Hopkins University (US)
- ORCID
- Gilman, Robert H.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2002-05
- Volume
- 40
- Issue
- 5
- Pages
- 1873-1874
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC130930
- OpenAlex
- W1977824993
- Cited by
- 119
- References
- 0
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 5 · 2020: 7 · 2019: 5 · 2018: 3 · 2017: 4 · 2016: 2 · 2015: 6 · 2014: 4 · 2013: 8 · 2012: 7
- FWCI
- 3.78
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- United States Agency for International Development
- National Institutes of Health
- Fogarty International Center
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Keywords
- Mycobacterium tuberculosis, antimicrobial susceptibility testing, multidrug-resistant tuberculosis, colorimetric assay, low-cost diagnostics
- MeSH terms
- Anti-Bacterial Agents, Colorimetry, Costs and Cost Analysis, Microbial Sensitivity Tests, Mycobacterium tuberculosis, Peru, Reproducibility of Results
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