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Detection and Characterization of the Hemolysin Genes in Aeromonas hydrophila and Aeromonas sobria by Multiplex PCR
Open accessAbstract
A multiplex PCR assay was designed to amplify the Aeromonas hydrophila and A. veronii bv. sobria hemolysin and aerolysin genes. The assay was evaluated by using 121 clinical isolates and 7 reference strains of Aeromonas spp., and these were divided into five genotypes on the basis of the results of the multiplex PCR. The five genotypes were characterized as type 1 for those carrying the ahh1 gene only (36% of isolates), type 2 for those carrying the asa1 gene only (8.5% of isolates), type 3 for those carrying both the ahh1 and the asa1 genes (4% of isolates), type 4 for those carrying the ahh1 gene and the A. hydrophila aerA (aerolysin) gene (37.5% of isolates), and type 5 for those in which no hemolysin genes were detected (14% of isolates). The most common single hemolysin gene carried among all the Aeromonas isolates examined was ahh1 , with 99 of 128 (77%) of isolates testing positive for this gene either alone or in combination with other hemolysin genes. Phenotypic expression of toxins was evaluated in a Vero cell culture cytotoxicity assay. These results indicated that there is a statistically significant correlation between the cytotoxin titers and the hemolysin genotype. Isolates belonging to genotype 4 (carrying both the ahh1 gene and the aerolysin and hemolysin aerA genes) expressed higher cytotoxin titers than isolates of the other genotypes ( P < 0.001). These isolates were more cytotoxic in cell culture and may have greater clinical significance.
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Citations
Cited by
The Genus Aeromonas : Taxonomy, Pathogenicity, and Infection
Janda, J. M., & Abbott, S. L. (2010) · Clinical Microbiology Reviews · doi:10.1128/cmr.00039-09 · Cited by 1,766
Virulence Factors of Aeromonas hydrophila: In the Wake of Reclassification
Rasmussen-Ivey, C. R., Figueras, M. J., McGarey, D., & Liles, M. R. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.01337 · Cited by 316
Metadata
- Authors
- Wang, G., Clark, C. G., Liu, C., Pucknell, C., Munro, C. K., Kruk, T. M. A. C., Caldeira, R., Woodward, D. L., & Rodgers, F. G.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2003-03
- Volume
- 41
- Issue
- 3
- Pages
- 1048-1054
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2131829531
- Cited by
- 228
- References
- 0
- FWCI
- 0.62
- Type
- journal article
- Language
- en
- Open access
- green
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- Wang, G., Clark, C. G., Liu, C., Pucknell, C., Munro, C. K., Kruk, T. M. A. C., Caldeira, R., Woodward, D. L., & Rodgers, F. G. (2003). Detection and Characterization of the Hemolysin Genes in Aeromonas hydrophila and Aeromonas sobria by Multiplex PCR. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.41.3.1048-1054.2003
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