Cited by 234Bacterial Identification and Susceptibility TestingBacterial Infections and VaccinesInfectious Diseases and Tuberculosis

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Prospective Study of Use of PCR Amplification and Sequencing of 16S Ribosomal DNA from Cerebrospinal Fluid for Diagnosis of Bacterial Meningitis in a Clinical Setting

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Schuurman, Tim, de Boer, Richard F., Kooistra-Smid, Anna M. D., van Zwet, Anton A.·2004·Journal of Clinical Microbiology

DOI 10.1128/jcm.42.2.734-740.2004PMC PMC344470

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Abstract

We have evaluated the use of a broad-range PCR aimed at the 16S rRNA gene in detecting bacterial meningitis in a clinical setting. To achieve a uniform DNA extraction procedure for both gram-positive and gram-negative organisms, a combination of physical disruption (bead beating) and a silica-guanidiniumthiocyanate procedure was used for nucleic acid preparation. To diminish the risk of contamination as much as possible, we chose to amplify almost the entire 16S rRNA gene. The analytical sensitivity of the assay was approximately 1 x 10(2) to 2 x 10(2) CFU/ml of cerebrospinal fluid (CSF) for both gram-negative and gram-positive bacteria. In a prospective study of 227 CSF samples, broad-range PCR proved to be superior to conventional methods in detecting bacterial meningitis when antimicrobial therapy had already started. Overall, our assay showed a sensitivity of 86%, a specificity of 97%, a positive predictive value of 80%, and a negative predictive value of 98% compared to culture. We are currently adapting the standard procedures in our laboratory for detecting bacterial meningitis; broad-range 16S ribosomal DNA PCR detection is indicated when antimicrobial therapy has already started at time of lumbar puncture or when cultures remain negative, although the suspicion of bacterial meningitis remains.

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Nucleic acid techniques in bacterial systematics
Stackebrandt, E. S., & Goodfellow, M. (1991) · Wiley eBooks · Cited by 9,460
16S/23S rRNA sequencing
Lane, D. (1991) · Medical Entomology and Zoology · Cited by 8,190
Rapid and simple method for purification of nucleic acids
Boom, R., Sol, C. J. A., Salimans, M. M. M., Jansen, C. L., Dillen, P. M. E. W., & van der Noordaa, J. (1990) · Journal of Clinical Microbiology · doi:10.1128/jcm.28.3.495-503.1990 · Cited by 5,285
The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy
Cole, J. R. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg039 · Cited by 1,435
Acute Bacterial Meningitis in Adults -- A Review of 493 Episodes
Durand, M. L., Calderwood, S. B., Weber, D. J., Miller, S. I., Southwick, F. S., Caviness, V. S., & Swartz, M. N. (1993) · New England Journal of Medicine · doi:10.1056/nejm199301073280104 · Cited by 1,344
Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction
Wilson, K. H., Blitchington, R. B., & Greene, R. C. (1990) · Journal of Clinical Microbiology · doi:10.1128/jcm.28.9.1942-1946.1990 · Cited by 647
Simultaneous Detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in Suspected Cases of Meningitis and Septicemia Using Real-Time PCR
Corless, C. E., Guiver, M., Borrow, R., Edwards-Jones, V., Fox, A. J., & Kaczmarski, E. B. (2001) · Journal of Clinical Microbiology · doi:10.1128/jcm.39.4.1553-1558.2001 · Cited by 598
Contamination and Sensitivity Issues with a Real-Time Universal 16S rRNA PCR
Corless, C. E., Guiver, M., Borrow, R., Edwards-Jones, V., Kaczmarski, E., & Fox, A. J. (2000) · Journal of Clinical Microbiology · doi:10.1128/jcm.38.5.1747-1752.2000 · Cited by 455
Identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci
Facklam, R. R., & Elliott, J. A. (1995) · Clinical Microbiology Reviews · doi:10.1128/cmr.8.4.479 · Cited by 445
Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation
Brisson-Noël, A., Nguyen, S. V., Aznar, C., Chureau, C., Garrigue, G., Pierre, C., Bartoli, M., Bonète, R., Pialoux, G., & Gicquel, B. (1991) · The Lancet · doi:10.1016/0140-6736(91)90492-8 · Cited by 302

Show all 63 references

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Busch, N., P. R. Mertens, T. Schonfelder, H. Nguyen, H. U. Marschall, H. Kierdorf, G. Haase, R. Urhahn, H. G. Sieberth, and S. Matern. 1996. Lemierre's post-tonsillitis sepsis with meningitis and intravascular consumption coagulopathy as complication of infectious mononucleosis with pansinusitis. Dtsch. Med. Wochenschr.121:94-98.
Vernazza, P. L., J. R. Dyer, S. A. Fiscus, J. J. Eron, and M. S. Cohen. 1997. HIV-1 viral load in blood, semen and saliva. AIDS11:1058-1059.

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Authors: Schuurman, T., de Boer, R. F., Kooistra-Smid, A. M. D., & van Zwet, A. A.
Affiliations: Department of Research and Development, Regional Public Health Laboratory for Groningen and Drenthe, Groningen, The Netherlands
Corresponding author: Schuurman, Tim
ORCID: Kooistra-Smid, Anna M. D.
Venue: Journal of Clinical Microbiology
Publisher: American Society for Microbiology
Published: 2004-02
Volume: 42
Issue: 2
Pages: 734-740
ISSN: 0095-1137 (ISSN-L)
1098-660X
DOI: 10.1128/jcm.42.2.734-740.2004
PMC: PMC344470
OpenAlex: W2070801207
Cited by: 234
References: 63
Citations by year: 2026: 9 · 2025: 6 · 2024: 7 · 2023: 8 · 2022: 5 · 2021: 11 · 2020: 14 · 2019: 13 · 2018: 13 · 2017: 7 · 2016: 10 · 2015: 12 · 2014: 8 · 2013: 13 · 2012: 12
FWCI: 6.51
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Journal of Clinical Microbiology (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
Health Sciences › Medicine › Infectious Diseases › Infectious Diseases and Tuberculosis
Keywords: bacterial meningitis, 16S rRNA gene, cerebrospinal fluid, broad-range PCR, molecular diagnosis, nucleic acid extraction, bead beating, guanidine thiocyanate, sensitivity and specificity, antimicrobial therapy, culture-negative meningitis
MeSH terms: Base Sequence, DNA, Ribosomal, Escherichia coli, Escherichia coli Infections, Gene Amplification, Humans, RNA, Ribosomal, 16S, Sensitivity and Specificity, Staphylococcal Infections, Reproducibility of Results, Polymerase Chain Reaction, Meningitis, Bacterial, DNA Primers

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Prospective Study of Use of PCR Amplification and Sequencing of 16S Ribosomal DNA from Cerebrospinal Fluid for Diagnosis of Bacterial Meningitis in a Clinical Setting

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