The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
New Real-Time PCR Assay for Rapid Detection of Methicillin- Resistant Staphylococcus aureus Directly from Specimens Containing a Mixture of Staphylococci
Open accessAbstract
Molecular methods for the rapid identification of methicillin-resistant Staphylococcus aureus(MRSA) are generally based on the detection of an S. aureus-specific gene target and the mecA gene. However, such methods cannot be applied for the direct detection of MRSA from nonsterile specimens such as nasal samples without the previous isolation, capture, or enrichment of MRSA because these samples often contain both coagulase-negative staphylococci (CoNS) and S. aureus, either of which can carry mecA.In this study, we describe a real-time multiplex PCR assay which allows the detection of MRSA directly from clinical specimens containing a mixture of staphylococci in <1 h. Five primers specific to the different staphylococcal cassette chromosome mec(SCC mec) right extremity sequences, including three new sequences, were used in combination with a primer and three molecular beacon probes specific to the S. aureus chromosomal orfX gene sequences located to the right of the SCC mec integration site. Of the 1,657 MRSA isolates tested, 1,636 (98.7%) were detected with the PCR assay, whereas 26 of 569 (4.6%) methicillin-susceptible S. aureus(MSSA) strains were misidentified as MRSA. None of the 62 nonstaphylococcal bacterial species or the 212 methicillin-resistant or 74 methicillin-susceptible CoNS strains (MRCoNS and MSCoNS, respectively) were detected by the assay. The amplification of MRSA was not inhibited in the presence of high copy numbers of MSSA, MRCoNS, or MSCoNS. The analytical sensitivity of the PCR assay, as evaluated with MRSA-negative nasal specimens containing a mixture of MSSA, MRCoNS, and MSCoNS spiked with MRSA, was ∼25 CFU per nasal sample. This real-time PCR assay represents a rapid and powerful method which can be used for the detection of MRSA directly from specimens containing a mixture of staphylococci.
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Cites
National Committee for Clinical Laboratory Standards
Bruck, E. (1980) · Pediatrics · doi:10.1542/peds.65.1.187 · Cited by 14,751
Cosgrove, S. E., Sakoulas, G., Perencevich, E. N., Schwaber, M. J., Karchmer, A. W., & Carmeli, Y. (2003) · Clinical Infectious Diseases · doi:10.1086/345476 · Cited by 1,964
Whole genome sequencing of meticillin-resistant Staphylococcus aureus
Kuroda, M., Ohta, T., Uchiyama, I., Baba, T., Yuzawa, H., Kobayashi, I., Cui, L., Oguchi, A., Aoki, K.-I., Nagai, Y., et al. (2001) · The Lancet · doi:10.1016/s0140-6736(00)04403-2 · Cited by 1,961
The Changing Epidemiology of Staphylococcus aureus?
Chambers, H. F. (2001) · Emerging Infectious Diseases · doi:10.3201/eid0702.010204 · Cited by 1,495
Genome and virulence determinants of high virulence community-acquired MRSA
Baba, T., Takeuchi, F., Kuroda, M., Yuzawa, H., Aoki, K.-I., Oguchi, A., Nagai, Y., Iwama, N., Asano, K., Naimi, T., et al. (2002) · The Lancet · doi:10.1016/s0140-6736(02)08713-5 · Cited by 1,370
Diekema, D. J., Pfaller, M. A., Schmitz, F. J., Smayevsky, J., Bell, J., Jones, R. N., Beach, M., & SENTRY Participants Group (2001) · Clinical Infectious Diseases · doi:10.1086/320184 · Cited by 1,362
Ledell, K., Muto, C. A., Jarvis, W. R., & Farr, B. M. (2003) · Infection Control & Hospital Epidemiology · doi:10.1086/502924 · Cited by 1,192
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Dissemination of New Methicillin-Resistant Staphylococcus aureus Clones in the Community
Okuma, K., Iwakawa, K., Turnidge, J. D., Grubb, W. B., Bell, J. M., O'Brien, F. G., Coombs, G. W., Pearman, J. W., Tenover, F. C., Kapi, M., et al. (2002) · Journal of Clinical Microbiology · doi:10.1128/jcm.40.11.4289-4294.2002 · Cited by 888
Cited by
García-Álvarez, L., Holden, M. T., Lindsay, H., Webb, C. R., Brown, D. F., Curran, M. D., Walpole, E., Brooks, K., Pickard, D. J., Teale, C., et al. (2011) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(11)70126-8 · Cited by 939
The evolution of Staphylococcus aureus
Deurenberg, R. H., & Stobberingh, E. E. (2008) · Infection, Genetics and Evolution · doi:10.1016/j.meegid.2008.07.007 · Cited by 721
Kowalski, T. J., Berbari, E. F., & Osmon, D. R. (2005) · Mayo Clinic Proceedings · doi:10.4065/80.9.1201 · Cited by 132
Donnio, P.-Y., Oliveira, D. C., Faria, N. A., Wilhelm, N., Le Coustumier, A., & de Lencastre, H. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.8.4191-4193.2005 · Cited by 82
Fang, C.-T., Shau, W.-Y., Hsueh, P.-R., Chen, Y.-C., Wang, J.-T., Hung, C.-C., Huang, L. Y. L., & Chang, S.-C. (2006) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkl006 · Cited by 53
Metadata
- Authors
- Huletsky, A., Giroux, R., Rossbach, V., Gagnon, M., Vaillancourt, M., Bernier, M., Gagnon, F., Truchon, K., Bastien, M., Picard, F. J., van Belkum, A., Ouellette, M., Roy, P. H., & Bergeron, M. G.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-05
- Volume
- 42
- Issue
- 5
- Pages
- 1875-1884
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2114764103
- Cited by
- 408
- References
- 44
- FWCI
- 19.48
- Type
- journal article
- Language
- en
- Open access
- green
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- Huletsky, A., Giroux, R., Rossbach, V., Gagnon, M., Vaillancourt, M., Bernier, M., Gagnon, F., Truchon, K., Bastien, M., Picard, F. J., van Belkum, A., Ouellette, M., Roy, P. H., & Bergeron, M. G. (2004). New Real-Time PCR Assay for Rapid Detection of Methicillin- Resistant Staphylococcus aureus Directly from Specimens Containing a Mixture of Staphylococci. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.42.5.1875-1884.2004
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
