Comparison of Flow Cytometric and Alamar Blue Tests with the Proportional Method for Testing Susceptibility of Mycobacterium tuberculosis to Rifampin and Isoniazid
Open accessAbstract
The performance of flow cytometry and the microplate Alamar Blue assay in determining susceptibility of Mycobacterium tuberculosis was assessed by testing 150 Brazilian isolates. The overall agreement was 97.3 and 98% for isoniazid and 94.7 and 100% for rifampin by flow cytometry and MABA, respectively. This study was entirely done in a developing country.
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Alamar blue dye (resazurin) is an effective growth indicator that can be used for MIC endpoint determination.
“The Alamar blue dye (resazurin), an effective growth indicator, can also be used for this purpose [61], [62], [63], [64].”
From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [61]
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Methods for in vitro evaluating antimicrobial activity: A review
Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016) · Journal of Pharmaceutical Analysis · doi:10.1016/j.jpha.2015.11.005 · Cited by 7,264 · see citing works
- Authors
- Reis, R. S., Neves, I., Lourenço, S. L. S., Fonseca, L. S., & Lourenço, M. C. S.
- Affiliations
- Fundação Oswaldo Cruz (BR)
- Oswaldo Cruz Fundation (FIOCRUZ)
- Universidade Federal do Rio de Janeiro (BR)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-05
- Volume
- 42
- Issue
- 5
- Pages
- 2247-2248
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC404654
- OpenAlex
- W2102656675
- Cited by
- 117
- References
- 0
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 3 · 2021: 5 · 2020: 8 · 2019: 2 · 2018: 2 · 2016: 3 · 2015: 4 · 2014: 6 · 2013: 8 · 2012: 9
- FWCI
- 3.19
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Arca - Repositório Institucional da Fiocruz (submittedVersion, other-oa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Keywords
- Mycobacterium tuberculosis, rifampicin, isoniazid, antimicrobial susceptibility testing, flow cytometry, Alamar Blue assay, microplate assay, proportional method, drug resistance, Brazilian isolates
- MeSH terms
- Antitubercular Agents, Brazil, Coloring Agents, Flow Cytometry, Humans, Isoniazid, Microbial Sensitivity Tests, Mycobacterium tuberculosis, Oxazines, Rifampin, Xanthenes
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