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Detection of Pneumocystis jiroveci in Respiratory Specimens by Four Staining Methods
Open accessAbstract
We examined four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination. The sensitivity and specificity of Calcofluor white stain (CW) were 73.8 and 99.6%, respectively. The sensitivity and specificity of Grocott-Gomori methenamine silver stain (GMS) were 79.4 and 99.2%, respectively. The sensitivity and specificity of Diff-Quik stain were 49.2 and 99.6%, respectively. The sensitivity and specificity of Merifluor Pneumocystis stain were 90.8 and 81.9%, respectively. Only CW and GMS had positive and negative predictive values of >90%.
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Cited by
Fishman, J. A., Gans, H., & Practice, T. A. I. D. C. O. (2019) · Clinical Transplantation · doi:10.1111/ctr.13587 · Cited by 293
Denning, D. W. (2016) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2015.0468 · Cited by 117
Pneumocystis Pneumonia: Current Concepts in Pathogenesis, Diagnosis, and Treatment
Krajicek, B. J., Thomas, C. F., & Limper, A. H. (2009) · Clinics in Chest Medicine · doi:10.1016/j.ccm.2009.02.005 · Cited by 73
Diagnosis and Management of Pneumocystis Pneumonia in Resource-poor Settings
Oladele, R. O., Otu, A. A., Richardson, M. D., & Denning, D. W. (2018) · Journal of Health Care for the Poor and Underserved · doi:10.1353/hpu.2018.0010 · Cited by 23
Metadata
- Authors
- Procop, G. W., Haddad, S., Quinn, J., Wilson, M. L., Henshaw, N. G., Reller, L. B., Artymyshyn, R. L., Katanik, M. T., & Weinstein, M. P.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-07
- Volume
- 42
- Issue
- 7
- Pages
- 3333-3335
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2152668642
- Cited by
- 164
- References
- 0
- FWCI
- 2.03
- Type
- journal article
- Language
- en
- Open access
- green
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- Procop, G. W., Haddad, S., Quinn, J., Wilson, M. L., Henshaw, N. G., Reller, L. B., Artymyshyn, R. L., Katanik, M. T., & Weinstein, M. P. (2004). Detection of Pneumocystis jiroveci in Respiratory Specimens by Four Staining Methods. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.42.7.3333-3335.2004
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.