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Detection of Pneumocystis jiroveci in Respiratory Specimens by Four Staining Methods

Open access
Procop, G. W., Haddad, S., Quinn, J., Wilson, M. L., Henshaw, N. G., Reller, L. B., Artymyshyn, R. L., Katanik, M. T., Weinstein, M. P.·2004·Journal of Clinical Microbiology

Abstract

We examined four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination. The sensitivity and specificity of Calcofluor white stain (CW) were 73.8 and 99.6%, respectively. The sensitivity and specificity of Grocott-Gomori methenamine silver stain (GMS) were 79.4 and 99.2%, respectively. The sensitivity and specificity of Diff-Quik stain were 49.2 and 99.6%, respectively. The sensitivity and specificity of Merifluor Pneumocystis stain were 90.8 and 81.9%, respectively. Only CW and GMS had positive and negative predictive values of >90%.

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Metadata

Authors
Procop, G. W., Haddad, S., Quinn, J., Wilson, M. L., Henshaw, N. G., Reller, L. B., Artymyshyn, R. L., Katanik, M. T., & Weinstein, M. P.
Venue
Journal of Clinical Microbiology
Published
2004-07
Volume
42
Issue
7
Pages
3333-3335
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
OpenAlex
W2152668642
Cited by
164
References
0
FWCI
2.03
Type
journal article
Language
en
Open access
green

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Procop, G. W., Haddad, S., Quinn, J., Wilson, M. L., Henshaw, N. G., Reller, L. B., Artymyshyn, R. L., Katanik, M. T., & Weinstein, M. P. (2004). Detection of Pneumocystis jiroveci in Respiratory Specimens by Four Staining Methods. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.42.7.3333-3335.2004

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.