Effect of Inoculum Size on Detection of Candida Growth by the BACTEC 9240 Automated Blood Culture System Using Aerobic and Anaerobic Media
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Simulated candidemia was produced with 20 Candida isolates at three inoculum sizes (100, 10, and 1 CFU/ml of blood). Growth detection was better with larger inocula. The time to growth detection was shorter with larger inocula. Inoculum size does effect Candida growth detection and time to detection in BACTEC 9240 automated systems.
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Detection of candidemia by blood culture often takes more than 24 hours, and for species such as Candida glabrata the time to positive culture can be even longer.
“Furthermore, detection of candidemia by blood culture often takes more than 24 hours.For certain species, such as C glabrata, the time to positive culture can be even longer.[56][57][58][59][60]”
From Fungal Sepsis: Optimizing Antifungal Therapy in the Critical Care Setting (2011) · cites this paper as [58]
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References 18 · Cited by 50
- Authors
- George, B. J., Horvath, L. L., & Hospenthal, D. R.
- Affiliations
- Brooke Army Medical Center (US)
- Corresponding author
- Horvath, Lynn L.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2005-01
- Volume
- 43
- Issue
- 1
- Pages
- 433-435
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC540153
- OpenAlex
- W2005548620
- Cited by
- 50
- References
- 18
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 5 · 2022: 5 · 2021: 2 · 2019: 2 · 2018: 3 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 3 · 2013: 5 · 2012: 3
- FWCI
- 1.50
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Keywords
- Candida, candidemia, inoculum size, BACTEC 9240, automated blood culture, detection time
- MeSH terms
- Aerobiosis, Anaerobiosis, Blood, Candida, Candida albicans, Candidiasis, Culture Media, Humans, Microbiological Techniques, Reagent Kits, Diagnostic, Fungemia
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