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Evaluation of 16S rRNA Sequencing and Reevaluation of a Short Biochemical Scheme for Identification of Clinically Significant Bacteroides Species
Open accessAbstract
Sequence analysis of the 16S rRNA gene represents a highly accurate and versatile method for bacterial classification and identification, even when the species in question is notoriously difficult to identify by phenotypic means. In this study, we evaluated the utility of 16S rRNA gene sequencing as a means of identifying clinically important Bacteroides species. We sequenced 231 clinical isolates that had been identified by a short biochemical scheme. Based on the sequence analysis, 192 clinical isolates were assigned to an established species, with the other 39 clinical strains revealing five unique sequences that may represent five novel species. This is in contrast to identification obtained from a short biochemical scheme, by which only 73.5% (172 of 231) of isolates were correctly identified to species level. Based on the solid identification obtained from 16S rRNA gene sequencing, the short biochemical scheme was modified and improved to provide clinical laboratories with an inexpensive and simple alternative for the identification of isolates of clinically significant Bacteroides species.
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16S rRNA sequencing is commonly used for bacterial identification.
“16S rRNA sequencing is commonly used for bacterial identification (Song et al., 2005)”
From The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain function in sepsis-associated encephalopathy (2023) · cites this paper as Song et al., 2005
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Li, Z., Zhang, F., Sun, M., Liu, J., Zhao, L., Liu, S., Li, S., & Wang, B. (2023) · PeerJ · doi:10.7717/peerj.15122 · Cited by 43
“16S rRNA sequencing is commonly used for bacterial identification ( Song et al., 2005 ), and the results revealed that there were no significant differences in gut microbiota diversity between the CLP group and the sham group.”
Metadata
- Authors
- Song, Y., Liu, C., Bolaňos, M., Lee, J., McTeague, M., & Finegold, S. M.
- Affiliations
- VA West Los Angeles Medical Center (US)
- Research Service
- Clinical Microbiology Laboratory
- University of California, Los Angeles (US)
- ORCID
- Liu, Chengxu
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2005-04
- Volume
- 43
- Issue
- 4
- Pages
- 1531-1537
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2087482996
- Cited by
- 43
- References
- 0
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 4 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 5 · 2014: 2 · 2012: 2
- FWCI
- 2.67
- Type
- journal article
- Language
- en
- Open access
- green
- dx.doi.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- Bacteroides, 16S rRNA gene sequencing, bacterial identification, novel bacterial species, clinical isolates, anaerobic bacteria, species identification
- MeSH terms
- Bacteroides, Bacteroides Infections, DNA, Ribosomal, Humans, Phenotype, Phylogeny, RNA, Ribosomal, 16S, Bacterial Typing Techniques, Polymerase Chain Reaction, Sequence Analysis, DNA, Genes, rRNA
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