Calcium Flux between the Endoplasmic Reticulum and Mitochondrion Contributes to Poliovirus-Induced Apoptosis
Open accessAbstract
We show that poliovirus (PV) infection induces an increase in cytosolic calcium (Ca 2+ ) concentration in neuroblastoma IMR5 cells, at least partly through Ca 2+ release from the endoplasmic reticulum lumen via the inositol 1,4,5-triphosphate receptor (IP 3 R) and ryanodine receptor (RyR) channels. This leads to Ca 2+ accumulation in mitochondria through the mitochondrial Ca 2+ uniporter and the voltage-dependent anion channel (VDAC). This increase in mitochondrial Ca 2+ concentration in PV-infected cells leads to mitochondrial dysfunction and apoptosis.
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Extra-mitochondrial Ca2+ enters the mitochondria via outer membrane voltage-dependent anion channels (VDACs) and the mitochondrial Ca2+ uniporter on the inner membrane.
“Extra-mitochondrial Ca2+ enters the mitochondria via outer membrane voltage-dependent anion channels (VDACs) and the mitochondrial Ca2+ uniporter on the inner membrane [71].”
From Neuronal Apolipoprotein E4 Expression Results in Proteome-Wide Alterations and Compromises Bioenergetic Capacity by Disrupting Mitochondrial Function (2019) · cites this paper as [71]
The Results and Discussion state that mitochondrial Ca2+ uptake occurs through VDAC and the Ca2+ uniporter, and report inhibition of uptake when either channel is blocked in poliovirus-infected IMR5 cells. Our copy does not explicitly locate VDAC in the outer membrane or the uniporter in the inner membrane, so it does not support that part of the sentence. (LLM, conf=0.82)
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Cites
Mitochondrial membrane permeabilization in cell death
Kroemer, G., Galluzzi, L., & Brenner, C. (2007) · Physiological Reviews · doi:10.1152/physrev.00013.2006 · Cited by 3,598 · see citing works
Self-citationMediators of endoplasmic reticulum stress-induced apoptosis
Szegezdi, E., Logue, S. E., Gorman, A. M., & Samali, A. (2006) · The EMBO Reports · doi:10.1038/sj.embor.7400779 · Cited by 2,345 · see citing works
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Research works printed but not linked: 5
- [13]13. Estaquier, J., T. Idziorek, F. de Bels, F. Barre-Sinoussi, B. Hurtrel, A. M. Aubertin, A. Venet, M. Mehtali, E. Muchmore, P. Michel, Y. Mouton, M. Girard, and J. C. Ameisen. 1994. Programmed cell death and AIDS: significance of T-cell apoptosis in pathogenic and nonpathogenic primate lentiviral infections. Proc. Natl. Acad. Sci. U. S. A. 91: 9431-9435.
- [18]18. Griffin, D. E. (ed.). 2005. Role of apoptosis in infection, vol. 289. Springer, Heidelberg, Germany.
- [26]26. Pallansch, M., and R. Roos. 2007. Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses, p. 839-893. In D. M. Knipe and P. M. Howley (ed.), Fields virology, vol. 1. Lippincott Williams and Wilkins, Philadelphia, PA.
- [27]27. Racaniello, V. R. 2007. Picornaviridae: the viruses and their replication, p. 795-838. In D. M. Knipe and P. M. Howley (ed.), Fields virology, 4th ed., vol. 1. Lippincott Williams and Wilkins, Philadelphia, PA.
- [31]31. Thastrup, O., P. J. Cullen, B. K. Drobak, M. R. Hanley, and A. P. Dawson. 1990. Thapsigargin, a tumor promoter, discharges intracellular Ca2 + stores by specific inhibition of the endoplasmic reticulum Ca2( + )-ATPase. Proc. Natl. Acad. Sci. U. S. A. 87: 2466-2470.
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Neurotropic Enterovirus Infections in the Central Nervous System
Huang, H.-I., & Shih, S.-R. (2015) · Viruses · doi:10.3390/v7112920 · Cited by 130 · see citing works
Orr, A. L., Kim, C., Jimenez-Morales, D., Newton, B. W., Johnson, J. R., Krogan, N. J., Swaney, D. L., & Mahley, R. W. (2019) · Journal of Alzheimer's Disease · doi:10.3233/jad-181184 · Cited by 103 · see citing works
- Authors
- Brisac, C., Téoulé, F., Autret, A., Pelletier, I., Colbère-Garapin, F., Brenner, C., Lemaire, C., & Blondel, B.
- Affiliations
- Inserm (FR)
- Institut Pasteur, Unité de Biologie des Virus Entériques, 25 rue du Dr Roux, 75015 Paris, France
- Corresponding author
- Blondel, Bruno
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2010-12
- Volume
- 84
- Issue
- 23
- Pages
- 12226-12235
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC2976416
- OpenAlex
- W2110448023
- Cited by
- 65
- References
- 2
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 5 · 2023: 1 · 2022: 4 · 2021: 4 · 2020: 2 · 2019: 6 · 2018: 2 · 2017: 6 · 2016: 2 · 2015: 8 · 2014: 6 · 2013: 5 · 2012: 1
- FWCI
- 1.21
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Virology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-09-MIEN-019, ANR-09-MIEN-0019)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
- Keywords
- poliovirus, apoptosis, calcium flux, endoplasmic reticulum, mitochondria, IP3 receptor, ryanodine receptor, mitochondrial calcium uniporter, voltage-dependent anion channel, mitochondrial dysfunction, neuroblastoma cells, viral infection
- MeSH terms
- Calcium, Cell Fractionation, Cytosol, Endoplasmic Reticulum, Flow Cytometry, Humans, Mitochondria, Poliomyelitis, Blotting, Western, Apoptosis, Poliovirus, Ryanodine Receptor Calcium Release Channel, Mitochondrial Diseases, Cell Line, Tumor, Inositol 1,4,5-Trisphosphate Receptors
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