The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus
Open accessAbstract
Vesicular stomatitis virions, Indiana serotype, were solubilized with high salt solubilizer and separated by ultracentrifugation into a supernatant fraction containing L, G, NS, and M proteins and pellet fraction containing the RNA complexed with N protein. NS protein was purified from the supernatnat fluid by sequential chromatography on phosphocellulose and diethylaminoethyl cellulose columns. The purified NS protein was assayed in a standard transcription system in combination with purified L protein and purified template (pellet fraction) prepared by renografin or CsCl banding. Results of the polymerase assays indicate that both L and NS proteins are required to reconstitute transcription activity with a highly purified template composed of only RNA and N protein. The NS protein polymerase activity is destroyed by trypsin but withstands 90 C temperatures for 10 min. Cytoplasmic NS protein can substitute for virion NS protein in the in vitro transcription assay.
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Cites
POLYRIBOSOMES IN NORMAL AND POLIOVIRUS-INFECTED HELA CELLS AND THEIR RELATIONSHIP TO MESSENGER-RNA
Penman, S., Scherrer, K., Becker, Y., & Darnell, J. E. (1963) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.49.5.654 · Cited by 463
Ribonucleic Acid Synthesis of Vesicular Stomatitis Virus, II. An RNA Polymerase in the Virion
Baltimore, D., Huang, A. S., & Stampfer, M. (1970) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.66.2.572 · Cited by 368
Emerson, S. U., & Wagner, R. R. (1972) · Journal of Virology · doi:10.1128/jvi.10.2.297-309.1972 · Cited by 229
Self-citationClassification of Rhabdovirus Proteins: a Proposal
Wagner, R. R., Prevec, L., Brown, F., Summers, D. F., Sokol, F., & MacLeod, R. (1972) · Journal of Virology · doi:10.1128/jvi.10.6.1228-1230.1972 · Cited by 189
Protein synthesis in vesicular stomatitis virus-infected HeLa cells
Mudd, J. A., & Summers, D. F. (1970) · Virology · doi:10.1016/0042-6822(70)90277-1 · Cited by 151
Huang, A. S.-H., & Manders, E. K. (1972) · Journal of Virology · doi:10.1128/jvi.9.6.909-916.1972 · Cited by 147
Huang, A., & Wagner, R. R. (1966) · Journal of Molecular Biology · doi:10.1016/0022-2836(66)90143-4 · Cited by 144
L Protein Requirement for In Vitro RNA Synthesis by Vesicular Stomatitis Virus
Emerson, S. U., & Wagner, R. R. (1973) · Journal of Virology · doi:10.1128/jvi.12.6.1325-1335.1973 · Cited by 138
Self-citationPhosphoproteins, structural components of rhabdoviruses
Sokol, F., & Clark, H. F. (1973) · Virology · doi:10.1016/0042-6822(73)90413-3 · Cited by 128
Bishop, D. H. L., & Roy, P. (1972) · Journal of Virology · doi:10.1128/jvi.10.2.234-243.1972 · Cited by 104
Cited by
Mühlberger, E., Weik, M., Volchkov, V. E., Klenk, H.-D., & Becker, S. (1999) · Journal of Virology · doi:10.1128/jvi.73.3.2333-2342.1999 · Cited by 486
Metadata
- Authors
- Emerson, S. U., & Yu, Y.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1975-06
- Volume
- 15
- Issue
- 6
- Pages
- 1348-1356
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1640479649
- Cited by
- 342
- References
- 25
- FWCI
- 20.72
- Type
- journal article
- Language
- en
- Open access
- green
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- Emerson, S. U., & Yu, Y. (1975). Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus. Journal of Virology. https://doi.org/10.1128/jvi.15.6.1348-1356.1975
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
