Monomer and multimer covalently closed circular forms of Rous sarcoma virus DNA
Open accessAbstract
Covalently closed circular molecules of viral DNA synthesized in virus-infected cells are composed mainly of monomers sedimenting at 22 to 27S in neutral sucrose gradients. These monomers are detected by annealing with complementary DNA or transfection assay. However, 11% of the infectious circles sediment faster than monomers. There is a peak at 32S which may correspond to dimer molecules. Traces of infectivity (about 3%) found between 32S and 65S suggest the presence of higher oligomers. In alkaline sucrose gradients, covalently closed monomers are found at 64 to 71S. Infectivity of these monomers is reduced by alkali treatment to less than one-tenth, and, perhaps for this reason, no infectious dimers or higher oligomers are observed. It has been shown that upon resedimentation the dimers of 95 can be separated from monomers and detected by hybridization.
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Mutations in specific amino acids of IN result in impaired integration of lentiviruses.
“However, several studies reported that mutations in specific amino acids of IN resulted in impaired integration of lentiviruses [8,26].”
From Non-Integrating Lentiviral Vectors in Clinical Applications: A Glance Through (2022) · cites this paper as [26]
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Wanisch, K., & Yáñez-Muñoz, R. J. (2009) · Molecular Therapy · doi:10.1038/mt.2009.122 · Cited by 222 · see citing works
Non-Integrating Lentiviral Vectors in Clinical Applications: A Glance Through
Gurumoorthy, N., Nordin, F., Tye, G. J., Wan Kamarul Zaman, W. S., & Ng, M. H. (2022) · Biomedicines · doi:10.3390/biomedicines10010107 · Cited by 69 · see citing works
- Authors
- Goubin, G., & Hill, M.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1979-02
- Volume
- 29
- Issue
- 2
- Pages
- 799-804
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC353215
- OpenAlex
- W1528683935
- Cited by
- 12
- References
- 0
- Citations by year
- 2022: 2
- FWCI
- 0.38
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Virology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- Rous sarcoma virus, viral DNA, covalently closed circular DNA, DNA dimers, DNA sedimentation, alkali treatment, DNA hybridization, viral DNA replication
- MeSH terms
- Animals, Avian Sarcoma Viruses, Chick Embryo, DNA, Circular, DNA, Viral, Nucleic Acid Conformation, Culture Techniques
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