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Cited by 70T-cell and Retrovirus StudiesInfectious Diseases and MycologyViral-associated cancers and disorders

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Transcription of mouse mammary tumor virus: identification of a candidate mRNA for the long terminal repeat gene product

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Wheeler, D A, Butel, J S, Medina, D, Cardiff, R D, Hager, G L·1983·Journal of Virology
DOI 10.1128/jvi.46.1.42-49.1983PMC PMC255091
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Abstract

We have examined an assortment of preneoplastic and neoplastic mouse mammary tissues for the presence of an mRNA which could encode the putative long terminal repeat gene product of mouse mammary tumor virus. We report here the detection of a novel mouse mammary tumor virus-specific, polyadenylic acid-containing transcript in certain preneoplastic and neoplastic mammary tissue of BALB/c mice. The molecule is 1.6 kilobases in length and contains sequences from the transcriptional leader and the U3 region of the proviral DNA. The upstream terminus of the 3' information lies 75 to 80 nucleotides from the beginning of the long terminal repeat open reading frame, in close proximity to a consensus splice acceptor in the DNA. The transcript was detected in hormonally or chemically induced neoplastic, preneoplastic, and lactating mammary tissue of BALB/c mice, but not in preneoplastic or tumor tissue induced by exogenous viruses in any strain of mice examined. This implies that the RNA we observed is transcribed from an endogenous provirus template.

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  • The major MMTV promoter within the env gene is constitutively active, unlike the major U3 promoter that is hormonally regulated; it is located downstream of about one-third of the env start site and can only transcribe the sag gene from a novel spliced mRNA.

    “The major MMTV promoter within the env gene is constitutively active, unlike the major U3 promoter that is hormonally regulated. It is located downstream of about one-third of the env start site and can only transcribe the sag gene from a novel spliced mRNA (Fig. 4C) (Wheeler et al. 1983, Jarvis et al. 1994, Mustafa et al. 2000).”

    From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Wheeler et al. 1983

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Research works printed but not linked: 39

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Metadata

Authors
Wheeler, D. A., Butel, J. S., Medina, D., Cardiff, R. D., & Hager, G. L.
Affiliations
National Institutes of Health (US)
ORCID
Venue
Journal of Virology
Publisher
American Society for Microbiology
Published
1983-04
Volume
46
Issue
1
Pages
42-49
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
DOI
10.1128/jvi.46.1.42-49.1983
PMC
PMC255091
OpenAlex
W2099841109
Cited by
70
References
0
Citations by year
2015: 1
FWCI
5.00
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Virology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
  • Health Sciences › Medicine › Infectious Diseases › Infectious Diseases and Mycology
  • Health Sciences › Medicine › Oncology › Viral-associated cancers and disorders
Keywords
mouse mammary tumor virus, long terminal repeat, mRNA synthesis, polyadenylation, proviral DNA, U3 region, mammary tumors, BALB/c mice, lactation
MeSH terms
Animals, DNA, Viral, Female, Genes, Viral, Mammary Tumor Virus, Mouse, Mammary Neoplasms, Experimental, Mice, Inbred Strains, Nucleic Acid Hybridization, Repetitive Sequences, Nucleic Acid, RNA Splicing, RNA, Messenger, RNA, Viral, Tissue Distribution, Transcription, Genetic, Mice

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