Expression from the adeno-associated virus p5 and p19 promoters is negatively regulated in trans by the rep protein
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The leftward two promoters of the adeno-associated virus (AAV) 2 genome were fused to reporter genes, and the constructs were used to transfect HeLa cells. The promoters functioned constitutively but were repressed in trans by the AAV rep gene product(s). The repression was relieved by adenovirus infection. Evidence which indicated an enhancer function for the inverted terminal repeat of the AAV-2 genome was also obtained.
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In the absence of a helper virus when Rep78 is acting to auto-suppress transcription from the p5 promoter, the sequence reads from the ITR region were higher than those from p5.
“In the absence of a helper virus when Rep78 is acting to auto-suppress transcription from the p5 promoter,40 the sequence reads from the ITR region were actually higher than those from p5,39”
From Adeno-Associated Virus Serotype-Specific Inverted Terminal Repeat Sequence Role in Vector Transgene Expression (2020) · cites this paper as [40]
Cites
Samulski, R. J., Srivastava, A., Berns, K. I., & Muzyczka, N. (1983) · Cell · doi:10.1016/0092-8674(83)90342-2 · Cited by 297 · see citing works
Self-citationTratschin, J. D., West, M. H., Sandbank, T., & Carter, B. J. (1984) · Molecular and Cellular Biology · doi:10.1128/mcb.4.10.2072 · Cited by 202 · see citing works
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Gene Expression from Adeno-associated Virus Vectors in Airway Epithelial Cells
Flotte, T. R., Solow, R., Owens, R. A., Afione, S., Zeitlin, P. L., & Carter, B. J. (1992) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb/7.3.349 · Cited by 167 · see citing works
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Sequence requirements for binding of Rep68 to the adeno-associated virus terminal repeats
Ryan, J. H., Zolotukhin, S., & Muzyczka, N. (1996) · Journal of Virology · doi:10.1128/jvi.70.3.1542-1553.1996 · Cited by 119 · see citing works
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- Authors
- Beaton, A., Palumbo, P., & Berns, K. I.
- Affiliations
- Cornell University (US)
- ORCID
- Palumbo, P
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1989-10
- Volume
- 63
- Issue
- 10
- Pages
- 4450-4454
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC251068
- OpenAlex
- W1857324335
- Cited by
- 160
- References
- 2
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 1 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 3 · 2013: 5
- FWCI
- 4.62
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Virology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (AI-22251)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Infectious Diseases › Parvovirus B19 Infection Studies
- Keywords
- adeno-associated virus type 2, transrepression, inverted terminal repeats, enhancer function, adenovirus infection, reporter gene expression, viral gene expression regulation
- MeSH terms
- Dependovirus, Gene Expression Regulation, Promoter Regions, Genetic, Transcription, Genetic, Viral Proteins, Chloramphenicol O-Acetyltransferase
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