The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Coinfection with recombinant vaccinia viruses expressing poliovirus P1 and P3 proteins results in polyprotein processing and formation of empty capsid structures
Open accessAbstract
The assembly process of poliovirus occurs via an ordered proteolytic processing of the capsid precursor protein, P1, by the virus-encoded proteinase 3CD. To further delineate this process, we have isolated a recombinant vaccinia virus which expresses, upon infection, the poliovirus P1 capsid precursor polyprotein with an authentic carboxy terminus. Coinfection of HeLa cells with the P1-expressing vaccinia virus and with a second recombinant vaccinia virus which expresses the poliovirus proteinase 3CD resulted in the correct processing of P1 to yield the three individual capsid proteins VP0, VP3, and VP1. When extracts from coinfected cells were fractionated on sucrose density gradients, the VP0, VP3, and VP1 capsid proteins were immunoprecipitated with type 1 poliovirus antisera from fractions corresponding to a sedimentation consistent for poliovirus 75S procapsids. Examination of these fractions by electron microscopy revealed structures which lacked electron-dense cores and which corresponded in size and shape to those expected for poliovirus empty capsids. We conclude that the expression of the two poliovirus proteins P1 and 3CD in coinfected cells is sufficient for the correct processing of the capsid precursor to VP0, VP3, and VP1 as well as for the assembly of poliovirus empty capsid-like structures.
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“In recent years, PV VLPs with native antigenicity have been produced in various systems, including mammalian cells, plants, insects, bacteria and yeast [129,131,132,133,134,135,136].”
From The Fight against Poliovirus Is Not Over (2023) · cites this paper as [131]
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Cites
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A second virus-encoded proteinase involved in proteolytic processing of poliovirus polyprotein
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Protein 3CD is the major poliovirus proteinase responsible for cleavage of the p1 capsid precursor
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Poliovirus proteinase 2A induces cleavage of eucaryotic initiation factor 4F polypeptide p220
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Further evidence on the formation of poliovirus proteins
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ANTIGEN SAMPLING ACROSS EPITHELIAL BARRIERS AND INDUCTION OF MUCOSAL IMMUNE RESPONSES
Neutra, M. R., Pringault, E., & Kraehenbühl, J.-P. (1996) · Annual Review of Immunology · doi:10.1146/annurev.immunol.14.1.275 · Cited by 475
Dougherty, W. G., & Semler, B. L. (1993) · Microbiological Reviews · doi:10.1128/mr.57.4.781-822.1993 · Cited by 346
Geller, R., Vignuzzi, M., Andino, R., & Frydman, J. (2007) · Genes & Development · doi:10.1101/gad.1505307 · Cited by 262
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Porta, C., Kotecha, A., Burman, A., Jackson, T., Ren, J., Loureiro, S., Jones, I. M., Fry, E. E., Stuart, D. I., & Charleston, B. (2013) · PLoS Pathogens · doi:10.1371/journal.ppat.1003255 · Cited by 156
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Shah, A. C., Benos, D. J., Gillespie, G. Y., & Markert, J. M. (2003) · Journal of Neuro-Oncology · doi:10.1023/b:neon.0000003651.97832.6c · Cited by 116
Metadata
- Authors
- Ansardi, D. C., Porter, D. C., & Morrow, C. D.
- Affiliations
- University of Alabama at Birmingham (US)
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1991-04
- Volume
- 65
- Issue
- 4
- Pages
- 2088-2092
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC240068
- OpenAlex
- W1535818125
- Cited by
- 64
- References
- 28
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 1
- FWCI
- 4.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Virology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (AI 07150, AI-25005, T32AI007150)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Immunology Research
- Life Sciences › Immunology and Microbiology › Virology › Poxvirus research and outbreaks
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Keywords
- poliovirus, co-infection, recombinant vaccinia virus, polyprotein processing, empty capsids, capsid assembly, VP0, VP3, VP1, HeLa cells
- MeSH terms
- 3C Viral Proteases, Base Sequence, Capsid, Cloning, Molecular, Cysteine Endopeptidases, DNA, Viral, Genes, Viral, HeLa Cells, Humans, Molecular Sequence Data, Poliomyelitis, Protein Processing, Post-Translational, Recombination, Genetic, Vaccinia virus, Viral Proteins, Gene Expression, Poliovirus, Capsid Proteins
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