The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Requirement of active human immunodeficiency virus type 1 integrase enzyme for productive infection of human T-lymphoid cells
Open accessAbstract
The human immunodeficiency virus type 1 (HIV-1) integrase enzyme exhibits significant amino acid sequence conservation with integrase proteins of other retroviruses. We introduced specific amino acid substitutions at a number of the conserved residue positions of recombinant HIV-1 integrase. Some of these substitutions resulted in proteins which were not able to be purified in the same manner as the wild-type enzyme, and these were not studied further. The remaining mutant enzymes were assessed for their abilities to perform functions characteristic of the integrase protein. These included specific removal of the terminal dinucleotides from oligonucleotide substrates representative of the viral U5-long terminal repeat, nonspecific cleavage of oligonucleotide substrates, and mediation of the strand transfer (integration) reaction. Substitution at position 43, within the protein's zinc finger motif region, resulted in an enzyme with reduced specificity for cleavage of the terminal dinucleotide. In addition, a double substitution of aspartic acid and glutamine for valine and glutamic acid, respectively, at positions 151 and 152 within the D,D(35)E motif region rendered the integrase protein inactive for all of its functions. The introduction of this double substitution into an infectious HIV-1 provirus yielded a mutant virus that was incapable of productively infecting human T-lymphoid cells in culture.
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1 retracted
Generation of deletion mutants of simian immunodeficiency virus incapable of proviral integration
Prakash, K., Ranganathan, P. N., Mettus, R. V., Reddy, P. S., Srinivasan, A., & Plotkin, S. A. (1993) · Journal of Virology · doi:10.1128/jvi.67.11.6916-.1993 · Cited by 8
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Vectors for selective expression of cloned DNAs by T7 RNA polymerase
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Active human immunodeficiency virus protease is required for viral infectivity
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Self-citationHIV-1 DNA integration: Mechanism of viral DNA cleavage and DNA strand transfer
Engelman, A., Mizuuchi, K., & Craigie, R. (1991) · Cell · doi:10.1016/0092-8674(91)90297-c · Cited by 655
Quiescent T Lymphocytes as an Inducible Virus Reservoir in HIV-1 Infection
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Cited by
Inhibitors of Strand Transfer That Prevent Integration and Inhibit HIV-1 Replication in Cells
Hazuda, D. J., Felock, P., Witmer, M., Wolfe, A., Stillmock, K., Grobler, J. A., Espeseth, A., Gabryelski, L., Schleif, W., Blau, C., et al. (2000) · Science · doi:10.1126/science.287.5453.646 · Cited by 1,131
Self-citationMultiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication
Engelman, A., Englund, G., Orenstein, J. M., Martin, M. A., & Craigie, R. (1995) · Journal of Virology · doi:10.1128/jvi.69.5.2729-2736.1995 · Cited by 445
Grobler, J. A., Stillmock, K., Hu, B., Witmer, M., Felock, P., Espeseth, A. S., Wolfe, A., Egbertson, M., Bourgeois, M., Melamed, J., et al. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.092056199 · Cited by 399
Espeseth, A. S., Felock, P., Wolfe, A., Witmer, M., Grobler, J., Anthony, N., Egbertson, M., Melamed, J. Y., Young, S., Hamill, T., et al. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.200139397 · Cited by 317
Metal Complexes as Enzyme Inhibitors
Louie, A. Y., & Meade, T. J. (1999) · Chemical Reviews · doi:10.1021/cr9804285 · Cited by 301
Metadata
- Authors
- LaFemina, R. L., Schneider, C. L., Robbins, H. L., Callahan, P. L., LeGrow, K., Roth, E., Schleif, W. A., & Emini, E. A.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1992-12
- Volume
- 66
- Issue
- 12
- Pages
- 7414-7419
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1846959000
- Cited by
- 259
- References
- 35
- FWCI
- 5.06
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Requirement of active human immunodeficiency virus type 1 integrase enzyme for productive infection of human T-lymphoid cells. https://app.cochat.ai/papers/10.1128/jvi.66.12.7414-7419.1992
- The paper
- LaFemina, R. L., Schneider, C. L., Robbins, H. L., Callahan, P. L., LeGrow, K., Roth, E., Schleif, W. A., & Emini, E. A. (1992). Requirement of active human immunodeficiency virus type 1 integrase enzyme for productive infection of human T-lymphoid cells. Journal of Virology. https://doi.org/10.1128/jvi.66.12.7414-7419.1992
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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