Optimal lengths for DNAs encapsidated by Epstein-Barr virus
Open accessAbstract
We measured the efficiency of DNA packaging by Epstein-Barr virus (EBV) as a function of the length of the DNA being packaged. Plasmids that contain oriP (the origin of latent EBV DNA replication), oriLyt (the origin of lytic EBV DNA replication), the viral terminal repeats (necessary for cleavage and packaging by EBV), and various lengths of bacteriophage lambda DNA were introduced into EBV-positive cells. Upon induction of the resident EBV's lytic phase, introduced plasmids replicated as concatemers and were packaged. Plasmid-derived concatemers of DNA with certain lengths were found to predominate in isolated virion particles. We measured the distribution of lengths of plasmid concatemers found within cells supporting the lytic phase of the viral life cycle and found that this distribution differed from the distribution of lengths of concatemers found in mature virion particles. This finding indicates that the DNA packaged into mature virions represents a selected subset of those present in the cell during packaging. These observations together indicate that the length of DNA affects the efficiency with which that DNA is packaged by EBV. Finally, we measured the length of the packaged B95-8 viral DNA and found it to be approximately 165 kbp, or 10 kbp shorter than the originally predicted size for B95-8 based on its sequence. Together with the results of other studies, these findings indicate that the packaging of DNAs by EBV is dependent on two imprecisely recognized elem…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Packaging capacity rarely exceeds 105 to 110% of the size of the wild-type genome.
“The above studies have found that packaging capacity rarely exceeds 105 to 110% of the size of the wt genome.”
From Packaging Capacity of Adeno-Associated Virus Serotypes: Impact of Larger Genomes on Infectivity and Postentry Steps (2005) · cites this paper as [3]
Cited by
Sorted by citations
Grieger, J. C., & Samulski, R. J. (2005) · Journal of Virology · doi:10.1128/jvi.79.15.9933-9944.2005 · Cited by 419 · see citing works
- Authors
- Bloss, T. A., & Sugden, B.
- Affiliations
- University of Wisconsin–Madison (US)
- ORCID
- Sugden, B
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1994-12
- Volume
- 68
- Issue
- 12
- Pages
- 8217-8222
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC237288
- OpenAlex
- W1805109123
- Cited by
- 57
- References
- 0
- Citations by year
- 2024: 2 · 2023: 1 · 2021: 1 · 2020: 3 · 2018: 2 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 5 · 2012: 2
- FWCI
- 1.20
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Virology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5p30ca007175-36)
- National Cancer Institute (CA-22443, CA-07175, T32CA009135, P01CA022443)
- Topics
- Health Sciences › Medicine › Oncology › Viral-associated cancers and disorders
- Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
- Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
- Keywords
- Epstein-Barr virus, DNA packaging, DNA length, concatemers, lytic replication, B95-8, bacteriophage lambda DNA, plasmid replication, packaging efficiency
- MeSH terms
- Animals, Callithrix, Capsid, Cell Line, Transformed, DNA Replication, DNA, Viral, Herpesvirus 4, Human, Molecular Weight, Plasmids, Repetitive Sequences, Nucleic Acid, Virion, Restriction Mapping, Replication Origin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
