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Three of the Four Nucleocapsid Proteins of Marburg Virus, NP, VP35, and L, Are Sufficient To Mediate Replication and Transcription of Marburg Virus-Specific Monocistronic Minigenomes

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Mühlberger, Elke, Lötfering, Beate, Klenk, Hans-Dieter, Becker, Stephan·1998·Journal of Virology
DOI 10.1128/jvi.72.11.8756-8764.1998
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Abstract

This paper describes the first reconstituted replication system established for a member of the Filoviridae, Marburg virus (MBGV). MBGV minigenomes containing the leader and trailer regions of the MBGV genome and the chloramphenicol acetyltransferase (CAT) gene were constructed. In MBGV-infected cells, these minigenomes were replicated and encapsidated and could be passaged. Unlike most other members of the order Mononegavirales, filoviruses possess four proteins presumed to be components of the nucleocapsid (NP, VP35, VP30, and L). To determine the protein requirements for replication and transcription, a reverse genetic system was established for MBGV based on the vaccinia virus T7 expression system. Northern blot analysis of viral RNA revealed that three nucleocapsid proteins (NP, VP35, and L) were essential and sufficient for transcription as well as replication and encapsidation. These data indicate that VP35, rather than VP30, is the functional homologue of rhabdo- and paramyxovirus P proteins. The reconstituted replication system was profoundly affected by the NP-to-VP35 expression ratio. To investigate whether CAT gene expression was achieved entirely by mRNA or in part by full-length plus-strand minigenomes, a copy-back minireplicon containing the CAT gene but lacking MBGV-specific transcriptional start sites was employed in the artificial replication system. This construct was replicated without accompanying CAT activity. It was concluded that the CAT activity reflected MBGV-specific transcription and not replication.

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Metadata

Authors
Mühlberger, E., Lötfering, B., Klenk, H.-D., & Becker, S.
Venue
Journal of Virology
Publisher
American Society for Microbiology
Published
1998-11-01
Volume
72
Issue
11
Pages
8756-8764
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
DOI
10.1128/jvi.72.11.8756-8764.1998
OpenAlex
W2160837638
Cited by
228
References
0
FWCI
3.21
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Three of the Four Nucleocapsid Proteins of Marburg Virus, NP, VP35, and L, Are Sufficient To Mediate Replication and Transcription of Marburg Virus-Specific Monocistronic Minigenomes. https://app.cochat.ai/papers/10.1128/jvi.72.11.8756-8764.1998
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Mühlberger, E., Lötfering, B., Klenk, H.-D., & Becker, S. (1998). Three of the Four Nucleocapsid Proteins of Marburg Virus, NP, VP35, and L, Are Sufficient To Mediate Replication and Transcription of Marburg Virus-Specific Monocistronic Minigenomes. Journal of Virology. https://doi.org/10.1128/jvi.72.11.8756-8764.1998

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