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Cited by 80Animal Virus Infections StudiesViral gastroenteritis research and epidemiologyvaccines and immunoinformatics approaches

The capsid gene of feline calicivirus contains linear B-cell epitopes in both variable and conserved regions

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Radford, Alan D., Willoughby, Kim, Dawson, Susan, McCracken, Christina, Gaskell, Rosalind M.·1999·Journal of Virology
DOI 10.1128/jvi.73.10.8496-8502.1999PMC PMC112869
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Abstract

In order to map linear B-cell (LBC) epitopes in the major capsid protein of feline calicivirus (FCV), an expression library containing random, short (100- to 200-bp) fragments of the FCV F9 capsid gene was constructed. Analysis of this library showed it to be representative of the region of the capsid gene that encodes the mature capsid protein. The library was screened by using polyclonal antisera from a cat that had been challenged experimentally with F9 to identify immunoreactive clones containing LBC epitopes. Twenty-six clones that reacted positively to feline antisera in immunoblots were identified. FCV-derived sequence from these clones mapped to a region of the capsid that spanned 126 amino acids and included variable regions C and E. An overlapping set of biotinylated peptides corresponding to this region was used to further map LBC epitopes by using F9 antisera. Four principal regions of reactivity were identified. Two fell within the hypervariable region at the 5′ end of region E (amino acids [aa] 445 to 451 [antigenic site {ags} 2] and aa 451 to 457 [ags 3]). However, the other two were in conserved regions (aa 415 to 421 [ags 1; region D] and aa 475 to 479 [ags 4; central region E]). The reactivity of the peptide set with antisera from 11 other cats infected with a range of FCV isolates was also determined. Ten of 11 antisera reacted to conserved ags 4, suggesting that this region may be useful for future recombinant vaccine design.

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  • The P2 domain plays a critical role in the viral infection cycle as it contains both the major immunodominant epitopes and the receptor-binding site.

    “However, the P2 domain plays a critical role in the viral infection cycle as it contains both the major immunodominant epitopes and the receptor-binding site [32, 35].”

    From Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures (2017) · cites this paper as [35]

    The Discussion identifies the 5′ hypervariable region of capsid region E as an important immunodominant region containing B-cell epitopes. It does not identify that region as the P2 domain or locate a receptor-binding site there; indeed, it says regions that interact with host proteins are largely unknown for caliciviruses. Thus, the epitope portion is supported, but the P2 and receptor-binding portions are not established by this paper. (LLM, conf=0.88)

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Authors
Radford, A. D., Willoughby, K., Dawson, S., McCracken, C., & Gaskell, R. M.
Affiliations
  • <!--label omitted: 1-->Department of Veterinary Pathology1 and
  • University of Liverpool (GB)
ORCID
Venue
Journal of Virology
Publisher
American Society for Microbiology
Published
1999-10
Volume
73
Issue
10
Pages
8496-8502
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
DOI
10.1128/jvi.73.10.8496-8502.1999
PMC
PMC112869
OpenAlex
W2136166839
Cited by
80
References
0
Citations by year
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FWCI
4.86
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Virology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
  • Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
Keywords
feline calicivirus, major capsid protein, linear B-cell epitopes, antigenic determinants, epitope mapping, hypervariable region, conserved domains, biotinylated peptides
MeSH terms
Amino Acid Sequence, Animals, Epitopes, B-Lymphocytes, Capsid, Cats, Molecular Sequence Data, Receptors, Antigen, B-Cell, Sequence Alignment, Conserved Sequence, Calicivirus, Feline

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