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Cited by 34Chromosomal and Genetic VariationsXenotransplantation and immune responseInfectious Diseases and Mycology

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Novel Endogenous Retrovirus in Rabbits Previously Reported as Human Retrovirus 5

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Griffiths, David J., Voisset, Cécile, Venables, Patrick J. W., Weiss, Robin A.·2002·Journal of Virology
DOI 10.1128/jvi.76.14.7094-7102.2002PMC PMC136318
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Abstract

Human retrovirus 5 (HRV-5) represented a fragment of a novel retrovirus sequence identified in human RNA and DNA preparations. In this study, the genome of HRV-5 was cloned and sequenced and integration sites were analyzed. Using PCR and Southern hybridization, we showed that HRV-5 is not integrated into human DNA. A survey of other species revealed that HRV-5 is present in the genomic DNA of the European rabbit ( Oryctolagus cuniculus ) and belongs to an endogenous retrovirus family found in rabbits. The presence of rabbit sequences flanking HRV-5 proviruses in human DNA extracts suggested that rabbit DNA was present in our human extracts, and this was confirmed by PCR analysis that revealed the presence of rabbit mitochondrial DNA sequences in four of five human DNA preparations tested. The origin of the rabbit DNA and HRV-5 in human DNA preparations remains unclear, but laboratory contamination cannot explain the preferential detection of HRV-5 in inflammatory diseases and lymphomas reported previously. This is the first description of a retrovirus genome in rabbits, and sequence analysis shows that it is related to but distinct from A-type retroelements of mice and other rodents. The species distribution of HRV-5 is restricted to rabbits; other species, including other members of the order Lagomorpha, do not contain this sequence. Analysis of HRV-5 expression by Northern hybridization and reverse transcriptase PCR indicates that the virus is transcribed at a low level in many rabbit tissues. In light of these findings we propose that the sequence previously designated HRV-5 should now be denoted RERV-H (for rabbit endogenous retrovirus H).

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  • Virus replication implies the generation of novel integration sites; any mechanism dependent on new proviral DNA must document such sites or risk the eventual discovery of a non-human origin for such sequences.

    “Virus replication implies the generation of novel integration sites; any mechanism dependent on new proviral DNA must document such sites or risk the embarrassment of an eventual discovery of a non-human origin for such sequences 93.”

    From Are human endogenous retroviruses pathogenic? An approach to testing the hypothesis (2013) · cites this paper as [93]

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Research works printed but not linked: 38

  1. 10.1101/gr.4.2.71 · DOI
  2. 10.1126/science.6189183 · DOI · Paper page
  3. 10.1128/jvi.27.2.313-319.1978 · DOI
  4. 10.1128/jvi.65.11.5663-5672.1991 · DOI
  5. 10.1006/jaut.1999.0307 · DOI
  6. 10.1093/oxfordjournals.molbev.a025818 · DOI
  7. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162 : 156-159.
  8. 10.1126/science.2425430 · DOI
  9. Garry, R. F., A. M. Krieg, W. P. Cheevers, R. C. Montelaro, H. Golding, C. D. Fermin, and W. R. Gallaher. 1995. Retroviruses and their roles in chronic inflammatory diseases and autoimmunity, p. 491-603. In J. A. Levy (ed.), The Retroviridae, vol. 4. Plenum Press, New York, N.Y.
  10. Gaudin, P., F. Moutet, P. W. Tuke, and J. A. Garson. 1999. Absence of human retrovirus 5 in French patients with rheumatoid arthritis. Arthritis Rheum. 42 : 2492-2494.
  11. 10.1002/1529-0131(200107)44:7<1689::AID-ART293>3.0.CO;2-K · DOI
  12. Griffiths D. J. 1996. Investigation of a novel retroviral element isolated from human salivary gland. Ph.D. thesis. University of London London United Kingdom.
  13. 10.1002/1529-0131(199904)42:3<448::AID-ANR9>3.0.CO;2-8 · DOI
  14. 10.1128/jvi.71.4.2866-2872.1997 · DOI
  15. Hunter, E., J. Casey, B. Hahn, M. Hayami, B. Korber, R. Kurth, J. Neil, A. Rethwilm, P. Sonigo, and J. Stoye. 2000. Retroviridae, p. 369-387. In M. H. van Regenmortel et al. (ed.), Virus taxonomy: the classification and nomenclature of viruses. Seventh report of the International Committee on Taxonomy of Viruses. Academic Press, San Diego, Calif.
  16. 10.1111/1523-1747.ep12505748 · DOI
  17. 10.1126/science.6981847 · DOI
  18. 10.1089/088922201750290069 · DOI
  19. 10.1016/S0065-230X(08)60223-7 · DOI
  20. 10.1006/geno.1993.1287 · DOI
  21. 10.1038/35057062 · DOI · Paper page
  22. Macdonald D. 2001. The new encyclopaedia of mammals. Oxford University Press Oxford United Kingdom.
  23. 10.1128/CMR.14.1.165-176.2001 · DOI
  24. 10.1006/geno.1995.9004 · DOI
  25. 10.1128/jvi.61.2.480-490.1987 · DOI · Paper page
  26. 10.1002/(SICI)1097-0215(20000315)85:6<762::AID-IJC4>3.0.CO;2-Z · DOI
  27. 10.1073/pnas.94.14.7583 · DOI
  28. 10.1073/pnas.77.12.7415 · DOI · Paper page
  29. 10.1016/S0002-9343(97)00344-6 · DOI
  30. 10.1002/art.1780401114 · DOI
  31. Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y.
  32. 10.1073/pnas.94.3.961 · DOI
  33. 10.1016/S0889-857X(05)70008-9 · DOI
  34. 10.1002/1529-0131(199906)42:6<1281::AID-ANR27>3.0.CO;2-8 · DOI
  35. 10.1016/0042-6822(87)90274-1 · DOI
  36. 10.1093/nar/22.22.4673 · DOI · Paper page
  37. 10.1126/science.1058040 · DOI · Paper page
  38. Weiss, R. A. 1984. The search for human RNA tumor viruses, p. 1205-1281. In R. Weiss, N. Teich, H. Varmus, and J. Coffin (ed.), RNA tumor viruses, 2nd ed., vol. 1. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

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Metadata

Authors
Griffiths, D. J., Voisset, C., Venables, P. J. W., & Weiss, R. A.
Affiliations
  • University College London (GB)
  • Wohl Virion Centre, Windeyer Institute of Medical Sciences, University College London, London W1T 4JF
  • Kennedy Institute of Rheumatology (GB)
Corresponding author
Griffiths, David J.
ORCID
Venue
Journal of Virology
Publisher
American Society for Microbiology
Published
2002-07-15
Volume
76
Issue
14
Pages
7094-7102
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
DOI
10.1128/jvi.76.14.7094-7102.2002
PMC
PMC136318
OpenAlex
W1972237923
Cited by
34
References
0
Citations by year
2026: 1 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 5 · 2014: 1 · 2013: 1 · 2012: 3
FWCI
3.38
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Virology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • UCL Discovery (University College London) (submittedVersion)
Funders
Abbott Laboratories
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
  • Health Sciences › Medicine › Surgery › Xenotransplantation and immune response
  • Health Sciences › Medicine › Infectious Diseases › Infectious Diseases and Mycology
Keywords
endogenous retroviruses, rabbits, proviral integration sites, lagomorphs
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Cats, Cell Line, Cloning, Molecular, Cricetinae, HeLa Cells, Humans, Molecular Sequence Data, Rabbits, Retroviridae, Retroviridae Infections, Virus Integration, Sequence Analysis, DNA, Endogenous Retroviruses, Mice

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