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Cited by 31Chemical Analysis and Environmental ImpactMicrobial bioremediation and biosurfactantsMicrobial Fuel Cells and Bioremediation

(Per)Chlorate-Reducing Bacteria Can Utilize Aerobic and Anaerobic Pathways of Aromatic Degradation with (Per)Chlorate as an Electron Acceptor

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Carlström, Charlotte I., Loutey, Dana, Bauer, Stefan, Clark, Iain C., Rohde, Robert A., Iavarone, Anthony T., Lucas, Lauren, Coates, John D.·2015·mBio
DOI 10.1128/mbio.02287-14PMC PMC4453571
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Abstract

The pathways involved in aromatic compound oxidation under perchlorate and chlorate [collectively known as (per)chlorate]-reducing conditions are poorly understood. Previous studies suggest that these are oxygenase-dependent pathways involving O 2 biogenically produced during (per)chlorate respiration. Recently, we described Sedimenticola selenatireducens CUZ and Dechloromarinus chlorophilus NSS, which oxidized phenylacetate and benzoate, two key intermediates in aromatic compound catabolism, coupled to the reduction of perchlorate or chlorate, respectively, and nitrate. While strain CUZ also oxidized benzoate and phenylacetate with oxygen as an electron acceptor, strain NSS oxidized only the latter, even at a very low oxygen concentration (1%, vol/vol). Strains CUZ and NSS contain similar genes for both the anaerobic and aerobic-hybrid pathways of benzoate and phenylacetate degradation; however, the key genes ( paaABCD ) encoding the epoxidase of the aerobic-hybrid phenylacetate pathway were not found in either genome. By using transcriptomics and proteomics, as well as by monitoring metabolic intermediates, we investigated the utilization of the anaerobic and aerobic-hybrid pathways on different electron acceptors. For strain CUZ, the results indicated utilization of the anaerobic pathways with perchlorate and nitrate as electron acceptors and of the aerobic-hybrid pathways in the presence of oxygen. In contrast, proteomic results suggest that strain NSS may use a combination of the anaerobic and aerobic-hybrid pathways when growing on phenylacetate with chlorate. Though microbial (per)chlorate reduction produces molecular oxygen through the dismutation of chlorite (ClO 2 − ), this study demonstrates that anaerobic pathways for the degradation of aromatics can still be utilized by these novel organisms. IMPORTANCE S. selenatireducens CUZ and D. chlorophilus NSS are (per)chlorate- and chlorate-reducing bacteria, respectively, whose genomes encode both anaerobic and aerobic-hybrid pathways for the degradation of phenylacetate and benzoate. Previous studies have shown that (per)chlorate-reducing bacteria and chlorate-reducing bacteria (CRB) can use aerobic pathways to oxidize aromatic compounds in otherwise anoxic environments by capturing the oxygen produced from chlorite dismutation. In contrast, we demonstrate that S. selenatireducens CUZ is the first perchlorate reducer known to utilize anaerobic aromatic degradation pathways with perchlorate as an electron acceptor and that it does so in preference over the aerobic-hybrid pathways, regardless of any oxygen produced from chlorite dismutation. D. chlorophilus NSS, on the other hand, may be carrying out anaerobic and aerobic-hybrid processes simultaneously. Concurrent use of anaerobic and aerobic pathways has not been previously reported for other CRB or any microorganisms that encode similar pathways of phenylacetate or benzoate degradation and may be advantageous in low-oxygen environments.

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  • Biological perchlorate reduction occurs primarily in anaerobic environments.

    “Additionally, biological perchlorate reduction occurs primarily in anerobic environments (Carlström et al., 2015).”

    From Simple and effective remediation strategies of Martian perchlorates (2026) · cites this paper as Carlström et al., 2015

    The Introduction describes bacteria that use perchlorate as an electron acceptor, and the Discussion describes anaerobic pathways of aromatic degradation during (per)chlorate reduction. Neither passage establishes that biological perchlorate reduction occurs primarily in anaerobic environments. Searches for anaerobic environments and anoxic perchlorate respiration found no statement about where reduction predominantly occurs. (LLM, conf=0.73)

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Authors
Carlström, C. I., Loutey, D., Bauer, S., Clark, I. C., Rohde, R. A., Iavarone, A. T., Lucas, L., & Coates, J. D.
Affiliations
  • University of California, Berkeley (US)
  • Energy Biosciences Institute, University of California, Berkeley, California, USA
  • Berkeley Earth (US)
Corresponding author
Coates, John D.
ORCID
Venue
mBio
Publisher
American Society for Microbiology
Published
2015-05
Volume
6
Issue
2
ISSN
  • 2150-7511 (ISSN-L)
  • 2161-2129
DOI
10.1128/mbio.02287-14
PMC
PMC4453571
OpenAlex
W2135277203
Cited by
31
References
0
Citations by year
2026: 2 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 2 · 2017: 3 · 2016: 3 · 2015: 1
FWCI
1.73
Type
journal article
Language
en
Open access
Licence
Locations
  • mBio (publishedVersion, cc-by-nc-sa)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by-nc-sa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • National Science Foundation (S10RR027303, S10RR029668)
  • Energy Biosciences Institute
  • National Institutes of Health
  • National Center for Research Resources (S10RR029668, S10RR027303)
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Chemical Analysis and Environmental Impact
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
  • Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
Keywords
aromatic compound degradation, electron acceptors, nitrate respiration
MeSH terms
Aerobiosis, Anaerobiosis, Chlorates, Hydrocarbons, Aromatic, Nitrates, Oxidation-Reduction, Oxygen, Perchlorates, Gammaproteobacteria, Metabolic Networks and Pathways

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