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Cited by 199RNA Research and SplicingRNA and protein synthesis mechanismsRNA regulation and disease

Analysis of Turnover and Translation Regulatory RNA-Binding Protein Expression through Binding to Cognate mRNAs

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Pullmann, Rudolf, Kim, Hyeon Ho, Abdelmohsen, Kotb, Lal, Ashish, Martindale, Jennifer L., Yang, Xiaoling, Gorospe, Myriam·2007·Molecular and Cellular Biology
DOI 10.1128/mcb.00500-07PMC PMC2099612
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Abstract

RNA-binding proteins (RBPs) that associate with specific mRNA sequences and function as mRNA turnover and translation regulatory (TTR) RBPs are emerging as pivotal posttranscriptional regulators of gene expression. However, little is known about the mechanisms that govern the expression of TTR-RBPs. Here, we employed human cervical carcinoma HeLa cells to test the hypothesis that TTR-RBP expression is influenced posttranscriptionally by TTR-RBPs themselves. Systematic testing of the TTR-RBPs AUF1, HuR, KSRP, NF90, TIA-1, and TIAR led to three key discoveries. First, each TTR-RBP was found to associate with its cognate mRNA and with several other TTR-RBP-encoding mRNAs, as determined by testing both endogenous and biotinylated transcripts. Second, silencing of individual TTR-RBPs influenced the expression of other TTR-RBPs at the mRNA and/or protein level. Third, further analysis of two specific ribonucleoprotein (RNP) complexes revealed that TIA-1 expression was controlled via HuR-enhanced mRNA stabilization and TIAR-repressed translation. Together, our findings underscore the notion that TTR-RBP expression is controlled, at least in part, at the posttranscriptional level through a complex circuitry of self- and cross-regulatory RNP interactions.

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  • HuD binds mRNAs of several RBPs, including its own, HuB, HuR, Cugbp2, Musashi 2, and Staufen 2, indicating a complex regulatory network between HuD and other components of the ribonome.

    “Of particular interest, this study also found that HuD binds mRNAs of several RBPs, including its own, HuB, HuR, Cugbp2, Musashi 2, and Staufen 2, indicating a complex regulatory network between HuD and other components of the ribonome (Pullmann et al. 2007; Mansfield and Keene 2009; Bolognani et al. 2010).”

    From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Pullmann et al. 2007

    The Abstract (page unavailable) says the study investigated AUF1, HuR, KSRP, NF90, TIA-1, and TIAR, and found binding among some of their mRNAs. Neither it nor the additional passages retrieved for HuD, Cugbp2, Musashi 2, and Staufen 2 reports the claimed HuD binding relationships. (LLM, conf=0.76)

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References 64 · Cited by 199

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Authors
Pullmann, R., Kim, H. H., Abdelmohsen, K., Lal, A., Martindale, J. L., Yang, X., & Gorospe, M.
Affiliations
National Institutes of Health (US)
Corresponding author
Gorospe, Myriam
ORCID
Venue
Molecular and Cellular Biology
Publisher
Informa UK Limited
Published
2007-09
Volume
27
Issue
18
Pages
6265-6278
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
DOI
10.1128/mcb.00500-07
PMC
PMC2099612
OpenAlex
W2073427046
Cited by
199
References
64
Citations by year
2026: 5 · 2025: 4 · 2024: 5 · 2023: 4 · 2022: 10 · 2021: 6 · 2020: 6 · 2019: 7 · 2018: 10 · 2017: 8 · 2016: 8 · 2015: 13 · 2014: 17 · 2013: 7 · 2012: 20
FWCI
6.76
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute on Aging
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords
RNA-binding proteins, mRNA turnover, translational regulation, post-transcriptional regulation, AUF1, HuR, KSRP, NF90, TIA-1, TIAR, mRNA stabilization, protein synthesis inhibition, ribonucleoprotein complexes, cross-regulation, HeLa cells
MeSH terms
T-Cell Intracellular Antigen-1, Gene Expression Regulation, HeLa Cells, Humans, Precipitin Tests, Protein Processing, Post-Translational, RNA, Messenger, Transfection, Protein Biosynthesis, Blotting, Western, RNA-Binding Proteins, Genes, Reporter, Biotinylation, 3' Untranslated Regions, RNA Interference, RNA, Small Interfering, Poly(A)-Binding Proteins, Green Fluorescent Proteins

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